Swallowing rehabilitation of patients undergoing T-tube implantation for the treatment of laryngotracheal stenosis.

Objective: Patients with laryngotracheal stenosis (LTS) often have dysphagia after laryngotracheal reconstruction with T-tube insertion, which affects the quality of life. The purpose of this study is to observe the effect of swallowing rehabilitation therapy on the improvement of quality of life in patients of otolaryngology-head and neck surgery with dysphagia undergoing T-tube implantation treatment through longitudinal study.

Methods: Thirty-eight patients with LTS who experienced dysphagia after laryngotracheal reconstruction and T-tube implantation were recruited.

Ginseng-derived nanoparticles inhibit lung cancer cell epithelial mesenchymal transition by repressing pentose phosphate pathway activity.

It is unclear whether ginseng-derived nanoparticles (GDNPs) can prevent tumor cell epithelial-mesenchymal transition (EMT). Here, we describe typical characteristics of GDNPs and possible underlying mechanisms for GDNP antitumor activities. First, GDNPs particle sizes and morphology were determined using nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM), respectively, while cellular uptake of PKH67-labeled GDNPs was also assessed.

Xianling Gubao Capsule Prevents Cadmium-Induced Kidney Injury.

Xianling Gubao Capsule (XGC), a kind of capsule preparation of Chinese herbal officially approved for sale by the National Medical Products Administration (NMPA), has the effect of tonifying kidney and strengthening bones. Although the impact of XGC in treating bone diseases has been widely studied, the effect of XGC in kidney injury is unknown yet. The kidney injury model is established by intraperitoneal injection with cadmium chloride (CdCl).

177 Saponins, Including 11 New Compounds in Wild Ginseng Tentatively Identified via HPLC-IT-TOF-MS, and Differences among Wild Ginseng, Ginseng under Forest, and Cultivated Ginseng.

Wild ginseng (W-GS), ginseng under forest (F-GS, planted in mountain forest and growing in natural environment), and cultivated ginseng (C-GS) were compared via HPLC-DAD and HPLC-IT-TOF-MS. A total of 199 saponins, including 16 potential new compounds, were tentatively identified from 100 mg W-GS (177 saponins in W-GS with 11 new compounds), F-GS (56 saponins with 1 new compound), and C-GS (60 saponins with 6 new compounds). There were 21 saponins detected from all the W-GS, F-GS, and C-GS.

Antifatigue Activity of Glycoprotein from Functions by Reducing Oxidative Stress.

The glycoprotein from was obtained with alkali extraction and acid precipitation, purified with DEAE Sepharose Fast Flow and Superdex G-75 column. The molecular composition structure and antifatigue activities of glycoprotein were studied. SCGP's molecular weight was approximately 10 KDa, and it consisted of a carbohydrate component (52.

Current Status and Problem-Solving Strategies for Ginseng Industry.

Ginseng is a plant in the family Araliaceae and the genus Panax with the formal name of Panax ginseng C. A. Meyer and the treasure of traditional herbal medicine resources as the "king of herbs".

Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation.

Adipose-derived stem cells (ADSCs) are multipotent and have received increasing attention for their applications in medicine. Cell-based therapies are optimal for diseases with loss or damage to tissues or organs. ADSCs and bone marrow mesenchymal stem cells (BMSCs) can differentiate into many cell lineages.

Chemical Constituents and Antioxidant, Anti-Inflammatory and Anti-Tumor Activities of Melilotus officinalis (Linn.) Pall.

Two new p-hydroxybenzoic acid glycosides, namely p-hydroxybenzoic acid-4--α-d-manopyranosyl-(1 → 3)-α-l-rhamnopyranoside (compound ) and 4--α-l-rhamnopyran-osyl-(1 → 6)-α-d-manopyranosyl-(1 → 3)-α-l-rhamnopyranoside (compound ), and seven known compounds, compound , , (acid components), compound , (flavonoids), compound (a coumarin) and compound (an alkaloid), were isolated from the 70% ethanol aqueous extract of the aerial parts of (Linn.) Pall. The structures of all compounds were elucidated by use of extensive spectroscopic methods Infrared Spectroscopy (IR), High resolution electrospray ionization mass spectrometry (HR-ESI-MS), and ¹H and C-NMR).

Ethyl acetate extract from Panax ginseng C.A. Meyer and its main constituents inhibit α-melanocyte-stimulating hormone-induced melanogenesis by suppressing oxidative stress in B16 mouse melanoma cells.

Ethnopharmacological Relevance: Hyperpigmentation disease involves darkening of the skin color due to melanin overproduction. Panax ginseng C.A.

Rapid characterization of ginsenosides in the roots and rhizomes of by UPLC-DAD-QTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS.

Background: Ginsenosides are the characteristic and principal components which manifest a variety of the biological and pharmacological activities of the roots and rhizomes of (GRR). This study was carried out to qualitatively and quantitatively determine the ginsenosides in the cultivated and forest GRR.

Methods: A rapid and sensitive ultra-high-performance liquid chromatography coupled with diode-array detector and quadrupole/time of flight tandem mass spectrometry (UPLC-DAD-QTOF-MS/MS) was applied to the qualitative analysis of ginsenosides and a 4000 QTRAP triple quadrupole tandem mass spectrometer (HPLC-ESI-MS) was applied to quantitative analysis of 19 ginsenosides.

Characterization and immunostimulating effects on murine peritoneal macrophages of a novel protein isolated from Panax quinquefolius L.

Ethnopharmacological Relevance: Panax quinquefolius L. has been used as a proverbial tonic in oriental countries for hundreds of years. It is used as a traditional medicinal herb to nourish vitality.

Anti-fatigue effects of proteins isolated from Panax quinquefolium.

Ethnopharmacological Relevance: American ginseng (Panax quinquefolium) is an obligate shade perennial plant that belongs to Araliaceae ginseng species, and is native to eastern USA and Canada. Ginseng proteins are reported to have several pharmaceutical properties. However, such properties of American ginseng proteins (AGP) have seldom been reported.

The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo.

This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFGF). Histological, immunohistochemistry and RT-PCR analysis confirmed that the ADSCs differentiated into chondrocytes following induction.

Nicotine addiction reduces the large-conductance Ca(2+)-activated potassium channels expression in the nucleus accumbens.

Large-conductance Ca(2+)-activated K(+) channels (BKCa) are widely expressed in the central nervous system and play important roles in neural activities. Nicotine exposure leads to long-lasting changes in behavioral and neuronal plasticity. However, little is known the roles of BKCa in the development of nicotine addiction.

A new chiral pyrrolyl α-nitronyl nitroxide radical attenuates β-amyloid deposition and rescues memory deficits in a mouse model of Alzheimer disease.

The generation of reactive oxygen species causes cellular oxidative damage, and has been implicated in the etiology of Alzheimer's disease (AD). L-NNNBP, a new chiral pyrrolyl α-nitronyl nitroxide radical synthesized in our department, shows potential antioxidant effects. The purpose of this study was to investigate the protective effects of L-NNNBP on β-amyloid (Aβ) deposition and memory deficits in an AD model of APP/PS1 mice.

[Raman spectroscopy study on the interaction of ginsenoside Rb1 with DPPC bilayers].

In the present work, the authors studied the interaction of ginsenoside Rh1 with lipid bilayers composed of DPPC using Raman spectroscopy. The conformational changes of DPPC molecule were further revealed by analyzing its vibrational modes such as the C--N stretching mode in the polar head-group region (650-850 cm(-1)), C--C stretching (1000-1200 cm(-1)), and C--H stretching (2750-3000 cm(-1)). The results indicated that there was little influence of Rb1 on the conformation of O--C--C--N+ backbone in the choline group of DPPC bilayers.

[Effects of deer tendons collagen on osteoporosis rats induced by retinoic acid].

Objective: To study the effects of deer tendons collagen on osteoporosis rats induced by retinoic acid.

Methods: Male Wistar rats were randomly divided into normal control group, model control group, deer tendons collagen high, medium and low-dose groups, osteoporosis rats of retinoic acid-induced were set up. Changes of body weight, bone weight, bone mineral density, bone histomorphometry, plasma phosphorus, calcium, alkaline phosphatase (ALP), bone mechanics were measured before and after treatment of deer tendons collagen.

[Structure and vibrational spectroscopy of ginsenoside Re: density functional theory study].

Density functional theory was used to optimize the geometry structure of two isomers of ginsenoside, Re, 20-(R)-R and 20-(S)-Re. The ginsenoside Re is an active constituent in ginseng. The calculated results show that there is an obvious difference in space structure between 20-(R)-R and 20-(S)-Re.

A new triterpene hexaglycoside from the bark of Kalopanax septemlobus (Thunb.) Koidz.

The new triterpene glycoside 3-O-beta-D-xylopyranosyl-(1-->4)-beta-D-xylopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosylhederagenin 28-O-beta-D-gluco-pyranosyl-(1-->6)-beta-D-glucopyranoside, named septemoside A (1), and the known 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-alpha-L-arabinopyranoside-28-O-beta-D-glucopyranosyl-(1-->6)-O-beta-D-glucopyranosyl ester of hederagenin (2), were isolated from the bark of Kalopanax septemlobus. The structure elucidation of the compounds was based on spectroscopic evidence, including HRESIMS, 1D and 2D-NMR analysis.

[Studies on the constituents from the herba of Erigeron acer].

Objective: To investigate the chemical constituents of the aerial part of Erigeron acer.

Methods: The chemical constituents were isolated by various columns and thin layer chromatographic methods. The structures were identified by spectral data.

[Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].

Objective: To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.

Method: The analy fical column was Shim-pack ODS - C18 (4.

[Isolation, cultivation of adipose-derived mesenchymal stem cells and its chondrogenic ability].

Aim: To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability.

Methods: Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium.

Purification and Characterization of A Phospholipase A(2) from Agkistrodon blomhoffii Ussurensis of Changbai Mountains.

PLA(2)(EC3.1.1.