[Overview of clinical occurrence of primary immunodeficiency disorders in children].

Objective: More than one hundred primary immunodeficiency disorders have been discovered so far. But the incidence of these disorders in our country is still not clear, so we analyzed the clinical data of 93 children with primary immunodeficiency disorders seen in our hospital in recent 30 years to understand the occurrence of primary immunodeficiency disorders in children, to promote the clinicians to become familiar with these disorders, to improve the nationwide registry system and to establish the basis for the treatment and prevention in future.

Methods: To analyze the constituent ratio of the 93 children with primary immunodeficiency disorders seen in our hospital from 1974 to 2003, diagnostic and classification criteria were set by taking the proposal by International Union of Immunological Societies (IUIS) PID classification committee in 2003 into account.

[Low dose methotrexate combined with GM-CSF induced differentiation of U937 cells].

Objective: To explore the effect of methotrexate on acute monoblastic leukemia cells, and the potential role of low-dose methotrexate in the treatment of monoblastic leukemia.

Methods: By using U937 cell line as a model, the induced differentiation of MTX or GM-CSF or their combination was detected by NBT reduction test, morphological observation and CD(14) expression. TRAP-ELISA was used for measuring telomerase activity.

[Primary study on establishment of namalwa 12/MTX resistant cell strain and its mechanism].

Background & Objective: Methotrexate has been used to treat malignant tumor for 50 years. Different mechanisms of methotrexate resistance has been known in various kinds of leukemias. Because of individualized chemotherapy according to different drug metabolism in different leukemia patients, the treatment became more reasonable and scientific and the treatment effect has been gradually improved.