A systematic analysis of the global and regional burden of colon and rectum cancer and the difference between early- and late-onset CRC from 1990 to 2019.

The burden of colorectal cancer (CRC) varies substantially across different geographical locations. However, there was no further quantitative analysis of regional social development and the disease burden of CRC. In addition, the incidence of early- and late-onset CRC has increased rapidly in developed and developing regions.

China's growing contribution to global intracranial aneurysm research (1991-2012): a bibliometric study.

Background: We sought to analyze the growing worldwide trends of intracranial aneurysm research, investigate China's recent contribution, and compare the contributions of mainland China, Taiwan, and Hong Kong.

Methods: Global and China intracranial aneurysm-related publications were retrieved from the Web of Science database from 1991 to 2012. Excel 2007, Matlab, and Thomson Data Analyzer (TDA) software were used to analyze the search results for number of publications, cited frequency, h-index, and organization contributions.

Status of anti-lung cancer drug patents applications in China from 2003 to 2012.

Background: During the past 10 years, dramatic progress has been made in the development of effective lung cancer drugs in China. However, little is known about their patents.

Objective: To summarize and analyze lung cancer patents or potential drug candidates issued in China over the last 10 years, and thus help researchers and developers to understand the current situation and potential candidates of lung cancer drug patents in China.

[Therapeutic effect of 125I-103Pd hybrid radioactive seeds on pulmonary carcinomas in BALB/c-nu mice].

Objective: To prepared (125)I-(103)Pd hybrid radioactive seeds and to explore their therapeutic effect on pulmonary carcinomas.

Methods: The (125)I-(103)Pd hybrid radioactive seeds were prepared by a chemical method of step-by-step coat plating. Pulmonary adenocarcinoma of GLC-82 cells and pulmonary large cell carcinoma of H460 cells were cultured in vitro and then were exposed directly to (125)I, (103)Pd and (125)I-(103)Pd seeds for 48 hours to observe the killing effects of radiation.

[Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants].

The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.).

[Isolation of 5' flanking region of the fruit-specific gene wml1 from watermelon by Uneven PCR].

The 5' flanking region of ADP-Glucose Pyrophosphorylase large subunit gene wml1, which covers 1864 bp, was isolated from watermelon genomic DNA by Uneven PCR. Sequence analysis indicated that there were putative TATA box and CAAT box at position 1237bp and 1284bp of the cloned fragment, respectively. Two transcriptional fusions between 5' regulational sequence of wml1 and beta-glucuronidase gene were constructed.

[Transformation of wml1 5' promoter region into tomato plants and studies on its transcriptional regulation role].

According to its restriction sites, fragments of 1573 bp, 1197 bp, 896 bp and 795 bp were obtained from the 5' promoter region of wml1 and fused with the coding sequence of the GUS gene. Constructs containing these fragments were introduced into tomato plants via Agrobacterium-mediated transformation. Histochemical assay of GUS expression in transgenic tomato plants revealed that fragments of 1573 bp, 1197 bp, 896 bp were able to direct GUS expression in fruits of 15, 30, 45 days after anthesis with the expression level of GUS increasing with fruit development, but not in leaves, stems and roots.