Exosomes Derived from Hydroquinone-transformed Human Bronchial Epithelial Cells Inhibited Recipient Cell Apoptosis by transferring miR-221.

Objective: Although benzene is a confirmed environmental carcinogen, the mechanism of its carcinogenicity remains largely unclear. The suggested oncogene, miR-221, is elevated and plays important roles in various tumors, but its role in benzene-induced carcinogenesis remains unknown.

Methods: In the present study, we constructed hydroquinone (HQ, a representative metabolite of benzene with biological activity)-transformed malignant cell line (16HBE-t) and analyzed the level of miR-221 in it with qRT-PCR.

[Epidemiological study on hyperuricemia and gout in Foshan areas, Guangdong province].

Objective: To determine the prevalence rates and risk factors of hyperuricemia (HUA) and gout among residents aged over 20 years in Foshan areas.

Methods: A randomly stratified cluster sampling was conducted, and 7403 inhabitants were investigated on their prevalence rates of HUA and gout.

Results: (1) The prevalence of HUA was 15.

Possible role of DNA polymerase beta in protecting human bronchial epithelial cells against cytotoxicity of hydroquinone.

Objective: To explore the toxicological mechanism of hydroquinone in human bronchial epithelial cells and to investigate whether DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone.

Methods: DNA polymerase beta knock-down cell line was established via RNA interference as an experimental group. Normal human bronchial epithelial cells and cells transfected with the empty vector of pEGFP-C1 were used as controls.

[Construction of vector of multiple loci gene targeting in leghorn chicken based on BAC with Cre/lox P system].

Based on the sequence of BAC (Bacterial Artificial Chromosome) along with the Cre/lox P system, the gene-targeting vectors to multiple loci of the repetitive internal transcribed spacers between rDNA genes in Leghorn chicken were constructed. The key material of multiple loci gene targeting in vivo would be obtained. First, the plasmid of pYLSV-TDN with TK, HRDS2, and Neo genes was constructed.

[hPARP1 genetic polymorphism in southern Chinese Han and Miao populations].

Objective: To study hPARP1 genetic polymorphism in southern Chinese Han and Miao populations.

Methods: Blood samples from 187 and 210 southern healthy Han and Miao populations were collected. The mutations of exons 12,13,16 and 17 of hPARP1 gene were investigated by PCR-single-strand conformation polymorphism(SSCP).

[Vector-mediated RNA interference of DNA polymerase beta in human bronchial epithelial cells].

Objective: To knock down the expression of polymerase beta gene in human bronchial epithelial cells with technology of vector-mediated RNA interference (RNAi), to provide research tool for the study on the functions and mechanisms of polymerase beta in repairing of DNA damaged by environmental chemical pollutants (ECPs).

Methods: Technology of molecular clone was used to construct the recombination vector of "pEGFP-C1-U6-dsRNA" for the polymerase beta RNAi. The recombinants were transfected into human bronchial epithelial cells with kit of liperfectamine 2000.

[Construction of short hairpin RNA vector of inhibiting poly ADP-ribose polymerase activity].

Objective: To construct expressing vector of short hairpin RNA (shRNA) in order to inhibit human PARP1 activity.

Methods: 2 pairs of 64 base oligos for hairpin RNA expression which targeted PARP1 gene were chemically synthesized and annealed then ligased with pSIREN-RetroQ vector with BamH II and EcoR I . Cut by EcoR t and Bgl II, shRNA and its upstream U6, which have 330 bp, were inserted into the same treated pEGFP-C1 vecter to construct GFP expression plasmids that inhibited hPARP1 protein shRNA plasmid (pEGFP-C1P).

[Construction of "pEGFP-C1-pU6-dsRNA" recombinant for human DNA polymerase beta RNA interference].

Objective: To clone the "pEGFP-C1-pU6-dsRNA" recombinant for human DNA polymerase beta RNA interference, to provide research tool for the study on the function of DNA polymerase beta in repairing of human DNA damaged by environmental chemical pollutants (ECPs).

Methods: According to the gene sequence of polymerase beta cDNA published in Genbank, double strand RNA(dsRNA) sequence which was used in RNA interference was designed by dsRNA oligonucleotide designer and synthesized by chemical methods. DNA recombination technology was used to insert the up related dsRNA sequence into the vector of pSIREN-RetroQ, and then the "pSIREN-RetroQ-dsRNA" recombinant was obtained.