Publications by authors named "Da-Yu Yang"
Article Synopsis
- The study developed a spectrum-effect relationship model for flavonoids in Citri Reticulatae Pericarpium (CRP), analyzing 15 batches of Liujunzi Decoction to identify effective components.
- HPLC analysis was performed using specific conditions to create chemical fingerprints of the flavonoids, leading to the discovery of key peaks that significantly influence gastrointestinal regulation.
- Findings indicated a linear correlation between the identified flavonoids and their effects, suggesting that the medicinal properties of CRP in Liujunzi Decoction arise from the combined action of multiple components.
Objective: To construct Epithelia Membrane Protein 1 gene-deficient in human fetal nucleus pulposus model by lentivirus-mediated RNA interference for building a platform for illustrating the biomechanisms role of EMP-1 during human intervertebral disc degeneration.
Methods: The lentivirus vector with shRNA targeting EMP-1 mRNA was transected into 293FT cells by liposome. Then the lentivirus supernatant was obtained and used for infecting human fetal nucleus pulposus.