CircATRNL1 protects against osteoarthritis by targeting miR-153-3p and KLF5.

Background: Osteoarthritis (OA) is characterized by chondrocyte injury. Circular RNAs (circRNAs) are involved in the pathogenesis of various diseases, including OA. The purpose of this study was to determine the potential role of circATRNL1 in OA pathology in vitro.

Comprehensive Effects of Suppression of MicroRNA-383 in Human Bone-Marrow-Derived Mesenchymal Stem Cells on Treating Spinal Cord Injury.

Background/aims: Transplantation of bone-marrow-derived mesenchymal stem cells (MSCs) promotes neural cell regeneration after spinal cord injury (SCI). Recently, we showed that suppression of microRNA-383 (miR-383) in MSCs increased the protein levels of glial cell line derived neurotrophic factor (GDNF), resulting in improved therapeutic effects on SCI. However, the overall effects of miR-383 suppression in MSCs on SCI therapy were not determined yet.

Suppression of MicroRNA-383 Enhances Therapeutic Potential of Human Bone-Marrow-Derived Mesenchymal Stem Cells in Treating Spinal Cord Injury via GDNF.

Background/aims: Transplantation of bone-marrow-derived mesenchymal stem cells (MSCs) has been used to treat spinal cord injury (SCI) to enhance tissue repair and neural cell regeneration. Glial cell line derived neurotrophic factor (GDNF) is an identified neural growth and survival factor. Here, we examined whether modification of GDNF levels in MSCs may further increase the potential of MSCs in promoting neural cell regeneration and subsequently the therapeutic outcome.

[Monitoring of Winter Wheat Aboveground Fresh Biomass Based on Multi-Information Fusion Technology].

The aim was to find a nondestructive way to improve the accuracy of detecting the winter wheat aboveground fresh biomass(AGFB). In this study, data fusion technology of the spectroscopy technology and the machine vision technology were used to analyze the AGFB and solve the problem that the accuracy of the prediction model of a single technology is not high. In this experiment, canopy spectra and canopy pictures of 93 samples at seeding stage were collected.

[Research on Grape Deterioration Process via Volatiles -Using Long Optical-Path Infrared Spectroscopy and Simplified E-Nose].

Grapes vaporize volatiles in specific compositions and concentrations during deterioration processes. Our previous study demonstrated that it is possible to analyze grapes spoilage stages by using the infrared spectra of their volatiles. However, only the spectral characteristics of alcohol, ethyl acetate and carbon dioxide were observed in the experiment because of the low concentration of the volatiles.

[A Study of FTIR Spectrometry Based on a Long Optical Path on the Emission Rules of Nitrous Oxide from Soil].

The excessive emission of N₂O (Nitrous oxide) will destroy the ozone layer, reasonable fertilization and adopting measures of emission reduction of N₂O are of great significance to slowing down the greenhouse effect. The article studied the impact of fertilization and water on the emission of N2 0 from the cabbage farmland using FTIR spectrometry. To enhance the sensitivity of the measuring system, we used multi-reflecting mirrors to increase the optical pathlength.

Actinomycin D inhibits cell proliferations and promotes apoptosis in osteosarcoma cells.

Actinomycin D (ActD), a well known transcription inhibitors, has been widely reported to induce cell apoptosis in several types of tumor cells by inhibiting the anti-apoptotic gene transcriptions. However, how ActD affects osteosarcoma cells survival and its molecular mechanism is currently unclear. In the present study, results of proliferation assays and Hoechst stainings suggested that MG63 human osteosarcoma cells showed impaired cell proliferations and significant apoptosis after ActD treatment.

[Research on error reduction of path change of liquid samples based on near infrared trans-reflective spectra measurement].

Based on sucrose solution as the research object, this paper measured the trans-reflective spectrum of sucrose solution of different concentration by the technique of near infrared spectrum in three optical path (4, 5, 6 mm). Five kinds of pretreatment method (vector normalization, baseline offset correction, multiplicative scatter correction, standard normal variate transformation, a derivative) were used to eliminate the influence of the optical path difference, and to establish model of the calibration set in combination with the PLS (Partial Least Squares)method. Five kinds of pretreatment method could restrain the inter ference of light path in varying degrees.

[Application of Fourier transform infrared spectroscopy in identification of wine spoilage].

In the present work, fresh and spoiled wine samples from three wines produced by different companies were studied u- sing Fourier transform infrared (FTIR) spectroscopy. We analyzed the physicochemical property change in the process of spoil- age, and then, gave out the attribution of some main FTIR absorption peaks. A novel determination method was explored based on the comparisons of some absorbance ratios at different wavebands although the absorbance ratios in this method were relative.

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold.

Background: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk's biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury.

[Pretreatment method of near-infrared diffuse reflection spectra used for sugar content prediction of pears].

The content of sugar is an important quality index for pears. However, the traditional sugar measurement methods are time-consuming and destructive. In the present study, the authors measured the sugar content of pears using visible and near infrared diffuse reflection spectroscopy.

Association of estrogen receptor alpha gene polymorphisms and risk of fracture.

The association between estrogen receptor alpha (ESR1) gene polymorphisms and risk of fracture is still controversial and ambiguous. The objective of this study was to evaluate the effect of PvuII polymorphisms of the ESR1 gene on fracture risk in Chinese patients. A population-based control study of elderly subjects was conducted in 120 fracture patients and 120 controls.

[Optimizing algorithm design of piecewise linear classifier for spectra].

Being able to identify pollutant gases quickly and accurately is a basic request of spectroscopic technique for envirment monitoring for spectral classifier. Piecewise linear classifier is simple needs less computational time and approachs nonlinear boundary beautifully. Combining piecewise linear classifier and linear support vector machine which is based on the principle of maximizing margin, an optimizing algorithm for single side piecewise linear classifier was devised.

[Gene expression profile in acute spinal cord injury screened by cDNA microarray: experiment with rats].

Objective: To investigate the characteristic changes of expression of the genes with specific functions in acute spinal cord injury (SCI).

Methods: Nine SD rats were randomly divided into 3 equal groups: SCI 8-hour group in which modified Allen's falling strike method was used to establish spinal cord contusion model, the spinal cords were taken out 8 hours later to undergo examination of the gene expression profile by using cDNA microarray including 13 200 gene, 12 genes were selected to undergo semi-quantitative RT-PCR, and the up-regulation of the candidate gene C/EBPdelta was verified by in situ hybridization and immunohistochemistry.; SCI 72-hour group undergoing the same treatment, however, with the spinal cord taken out 72 hours later; and control group undergoing only sham operation with the spinal cord taken out immediately.