[Cloning and expression of D-like aspartic protease of Anisakis simplex].

Objective: To clone and express the full length of D-like aspartic protease gene (AsAP) of the third stage larvae of Anisakis simplex.

Methods: According to the partial information of D-like aspartic protease encoding gene of A. simplex from GenBank, specific primers were designed to amplify 3'end and 5' end of AsAP gene using rapid amplification of cDNA ends (RACE), and the full length of the D-like aspartic protease gene was obtained.

[Detection of anisakid nematodes by an SYBR green I real-time PCR].

Objective: To establish an SYBR Green I real-time quantitative PCR method for the detection of anisakid nematodes with zoonotic potential from Taiwan Strait.

Methods: Anisakid larvae of six species (Anisakis simplex, A. physeteris, Raphidascaris trichiura, Contracaecum aduncum, C.

[Cloning and expression of L-like cysteine protease of Anisakis simplex].

Objective: To clone and express the full lenth of L-like cysteine protease gene of Anisakis simplex (AsCP).

Methods: According to L-like cysteine protease encoding gene of A. simplex from GenBank EST database, specific primers were designed to amplify 3'-end of AsCP gene using rapid-amplification of cDNA ends (RACE), and the full lenth of the L-like cysteine protease gene was obtained.

[Expressed sequence tags (ESTs) analysis of Angiostrongylus cantonensis].

A total of 1,277 ESTs of Angiostrongylus cantonensis were downloaded from GenBank and analyzed with BlastX. SignalP V3.0 analysis was applied to predict potential putative antigen or allergen relative proteins with N-terminal secreted signal peptides or signal anchors.

Purification, electrophoretic behavior, and kinetics of iron release of liver ferritin of Dasyatis akajei.

From the liver of fish Dasyatis akajei, ferritin has been isolated by thermal denaturation and ammonium sulfate fractionation and then further purified by anion exchange chromatography and gel exclusion chromatography. The molecular weight of the liver ferritin of D. akajei (DALF) was measured to be 400 kDa by PAGE.