3-Bromo-4,5-dihydroxybenzaldehyde Attenuates Allergic Contact Dermatitis by Generating CD4Foxp3 T cells.

Background/aim: Regulatory T cells (Tregs) play a crucial role in inflammatory responses by regulating the activity of various immune cells. M2 macrophages induced by IL-10 and TGF-β exhibit anti-inflammatory functions and induce Treg differentiation. Although the beneficial effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) on various diseases have been widely reported, the mechanisms, through which it alleviates allergic contact dermatitis (ACD) via Tregs and macrophages, are not well understood.

Expression of IL-7RαCX3CR1 CD8 T Cells and α4β7 Integrin Tagged T Cells Related to Mucosal Immunity in Children with Inflammatory Bowel Disease.

Purpose: The study aimed to investigate the recruiting of T lymphocytes including IL-7Rα CX3CR1 effector memory (EM) CD8 T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa.

Methods: Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively.

Mucosal Immunity Related to CD8 T Lymphocytes in Children with Gastritis.

Purpose: We investigated the role of CD8T cells as host immune factors in pediatric patients with gastritis.

Methods: Gastric mucosal tissue and blood samples were collected from 39 children, including 11 children with infection and 28 children as controls. Anti-CD8 and anti-T-bet antibodies were used for immunohistochemistry of the gastric mucosa.

Effector Memory CD8 and CD4 T Cell Immunity Associated with Metabolic Syndrome in Obese Children.

Purpose: We investigated the association of effector memory (EM) CD8 T cell and CD4 T cell immunity with metabolic syndrome (MS).

Methods: Surface and intracellular staining of peripheral blood mononuclear cells was performed. Anti-interleukin-7 receptor-alpha (IL-7Rα) and CX3CR1 antibodies were used to stain the subsets of EM CD8 T cells, while anti-interferon-gamma (IFN-γ), interleukin-17 (IL-17), and forkhead box P3 (FOXP3) antibodies were used for CD4 T cell subsets.

Mucosal Immunity Related to FOXP3 Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease.

Background: We aimed to investigate mucosal immunity related to forkhead box P3 (FOXP3) regulatory T (Treg) cells, T helper 17 (Th17) cells and cytokines in pediatric inflammatory bowel disease (IBD).

Methods: Mucosal tissues from terminal ileum and colon and serum samples were collected from twelve children with IBD and seven control children. Immunohistochemical staining was done using anti-human FOXP3 and anti-RORγt antibodies.