Front Immunol
December 2024
Guillain-Barré syndrome (GBS) is a heterogeneous disease, and it usually develops after an antecedent infection, while rare cases develop following a central nervous system disease. Given the indeterminacy of prodromal infection symptoms and the overlap of symptoms with other neurological disorders, a rigorous clinical and neurological examination in conjunction with history is necessary for early diagnosis and treatment of GBS. Here we present a rare case of GBS following acute ischemic stroke which was different from previous reports such as GBS following hemorrhagic stroke or head trauma.
View Article and Find Full Text PDFProgrammable epigenome editors modify gene expression in mammalian cells by altering the local chromatin environment at target loci without inducing DNA breaks. However, the large size of CRISPR-based epigenome editors poses a challenge to their broad use in biomedical research and as future therapies. Here, we present Robust ENveloped Delivery of Epigenome-editor Ribonucleoproteins (RENDER) for transiently delivering programmable epigenetic repressors (CRISPRi, DNMT3A-3L-dCas9, CRISPRoff) and activator (TET1-dCas9) as ribonucleoprotein complexes into human cells to modulate gene expression.
View Article and Find Full Text PDFAiming at the problem of overbrightness of the target simulator background for LED backlight panel illumination, a 5° aperture angle-matched collimated illumination method for the target simulator is proposed based on the study of the dark-state leakage of the LCD display device and the scattered stray light of the system. After simulation analysis the method can make the display contrast increase by 3.1 times and solve the problem of dark targets being drowned by the background bright light, which exists in the LED illumination target simulator.
View Article and Find Full Text PDFThis study investigated the multiple correlations among spectral simulation units based on digital micromirror device (DMD) spectral simulation, which leads to the problem that conventional spectral simulation methods such as PID control exhibit a low fitting accuracy or long fitting time in the spectral simulation of various targets. In this paper, a method of stellar spectrum simulation based on back propagation neural network-based PID (BP-PID) control is proposed to achieve high efficiency and high precision simulation of various spectral targets. The topology of the BP neural network was constructed based on the spectral modulation model of a DMD stellar spectrum simulation system, and the algorithm of the BP-PID control was designed.
View Article and Find Full Text PDFAiming at the problem that the error of multi-optical axis alignment is difficult to control in the simulation calibration test, a multi-optical axis dynamic compensation alignment method for the space environment simulation system is proposed. The effect of optical axis disturbance on the signal detection link was analyzed to obtain the alignment accuracy requirements, the optical axis alignment error compensation mechanism was established, the target simulation system with long pupil distance was designed, the alignment error transfer model based on topology structure was constructed, and the test prototype was built. The test results show that the static attitude alignment resolution is better than 3" and the position alignment resolution is better than 11µm.
View Article and Find Full Text PDFBackground: Alzheimer's disease (AD) is a common elderly disorder characterized by cognitive decline. Endoplasmic reticulum (ER) stress has been implicated in various neurodegenerative diseases, including AD. Stem cell factor (SCF) performs its biological functions by binding to and activating receptor tyrosine kinase c-Kit.
View Article and Find Full Text PDFBackground: Observational studies suggest that hypertension and epilepsy have a high co-occurrence, and antihypertensive medications may have impacts on the prevention and treatment of epilepsy. However, the directionality of causation between them is elusive.
Method: By leveraging genome-wide association studies (GWAS) summary data of each trait, we firstly performed bidirectional univariate Mendelian randomization (UVMR) to assess the strength and direction of the associations between pairs of traits, then multivariate MR (MVMR) was conducted to adjust for potential confounders in causalities.
Inherently chiral eight-membered rings embedded in tetraphenylene derivatives and hetero-analogues exhibit unique properties and allow diverse applications. A conceptually viable and straightforward approach to these frameworks is [4+4] cycloaddition, which still remains elusive. Herein, we describe the stereoselective cross-[4+4] cycloaddition of quinone methides (QMs), leading to the formation of oxa-analogues of tetraphenylene with exceptional chemo-, diastereo-, and enantioselectivity.
View Article and Find Full Text PDFIron death is a novel type of programmed cell death caused by excessive accumulation of iron-dependent lipid peroxidation products; however, the function of iron death during porcine oocyte maturation and embryo growth is poorly understood. This study was conducted to investigate the mechanism of ferric ammonium citrate (FAC) in regulating iron death in mature oocytes in vitro through the NRF2 signaling pathway, and subsequent embryonic development. The experiment was divided into four groups: 0 (control group), 2, 5, and 10 μM FAC.
View Article and Find Full Text PDFDisialoganglioside (GD2) is one of the most popular overexpressed antigens for tumor cell targeting. However, GD2-specific antibodies often show unintended targeting to GD2-expressing health-maintaining cells due to the comparable binding affinities both at physiological pH and in a slightly acidic tumor microenvironment (TME). In this work, an affinity-switchable zwitterionic PAMAM G5 dendrimer (G5-3S) is developed for selective binding to GD2 only in a slightly acidic TME.
View Article and Find Full Text PDFCoupling direct air capture (DAC) with methane (CH) production is a potential strategy for fuel production from the air. Here, we report a hybrid electro-thermochemical device for direct CH production from air. The proposed device features the cogeneration of carbon dioxide (CO) and hydrogen (H) in a single compartment via a bipolar membrane electrodialysis module, avoiding a separate water electrolyzer, followed by a thermochemical methanation reaction to produce CH.
View Article and Find Full Text PDFBile acid (BA) homeostasis is vital for various physiological processes, whereas its disruption underlies cholestasis. The farnesoid X receptor (FXR) is a master regulator of BA homeostasis via the ileal fibroblast growth factor (FGF)15/19 endocrine pathway, responding to postprandial or abnormal transintestinal BA flux. However, the de novo paracrine signal mediator of hepatic FXR, which governs the extent of BA synthesis within the liver in non-postprandial or intrahepatic cholestatic conditions, remains unknown.
View Article and Find Full Text PDFHuman ABC transporters ABCD1-3 are all localized on the peroxisomal membrane and participate in the β-oxidation of fatty acyl-CoAs, but they differ from each other in substrate specificity. The transport of branched-chain fatty acids from cytosol to peroxisome is specifically driven by ABCD3, dysfunction of which causes severe liver diseases such as hepatosplenomegaly. Here we report two cryogenic electron microscopy (cryo-EM) structures of ABCD3 bound to phytanoyl-CoA and ATP at resolutions of 2.
View Article and Find Full Text PDFBackground: The adverse effects of a Western diet on obesity and diabetes among reproductive-aged women pose a significant threat to the cardiovascular health of their offspring. Given the crucial role of glutathione metabolism and glutathione-related antioxidant defense systems in cardiovascular diseases through scavenging ROS and maintaining redox homeostasis, further exploration of their specific influence is imperative to develop therapeutic strategies for cardiomyopathy induced by a maternal Western diet.
Methods: We developed a prenatal maternal Western diet exposure model in C57/B6 mice to investigate cardiac morphology and function through histological analysis and echocardiography.
Objectives: This study aimed to investigate the epidemiological and drug resistance (DR) characteristics of extrapulmonary tuberculosis (EPTB) in South-Central China.
Methods: EPTB inpatients who were culture-positive for were retrospectively included in a study at a provincial TB hospital in Hunan, a province in South-Central China, from January 2013 to December 2021. Demographic, clinical, and drug susceptibility data were retrieved from TB treatment records.