Marinobacter sediminicola sp. nov. and Marinobacter xiaoshiensis sp. nov., Isolated from Coastal Sediment.

Two Gram-stain-negative, facultative anaerobic, rod-shaped, motile bacterial strains, designated F26243 and F60267 were isolated from coastal sediment in Weihai, China. Strains F26243 and F60267 were grown at 4-40 °C (optimum 33 °C), pH 7.0-9.

sp. nov. and sp. nov., isolated from marine sediment.

Two Gram-stain-negative, rod-shaped, non-motile, strictly aerobic strains, forming yellow colonies and designated F6058 and S2608, were isolated from marine sediment collected in Weihai, PR China. Both strains grow at 4-40 °C (optimum, 30-33 °C), pH 6.0-7.

Cultivating the uncultured: Harnessing the "sandwich agar plate" approach to isolate heme-dependent bacteria from marine sediment.

In the classical microbial isolation technique, the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for survival. In this study, we designed a simple coculture technique named the "sandwich agar plate method," which maintains microbial interactions throughout the isolation and pure culture processes. The total yield of uncultured species in sandwich agar plates based on eight helper strains was almost 10-fold that of the control group.

Cerina litoralis gen. nov., sp. nov., a novel potential polysaccharide degrading bacterium of the family Flavobacteriaceae, isolated from marine sediment.

The Gram-strain-negative, facultative anaerobic, chemoheterotrophic, short-rod-shaped, non-motile, forming yellow colonies strain, designated F89, was isolated from marine sediment of Xiaoshi Island, Weihai. Strain F89 grew at 15-37 °C (optimally at 28 °C), at pH 6.0-8.

Short-chain fatty acids (SCFAs) as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments.

Unlabelled: Many marine bacteria are difficult to culture because they are dormant, rare or found in low-abundances. Enrichment culturing has been widely tested as an important strategy to isolate rare or dormant microbes. However, many more mechanisms remain uncertain.

Leader peptide removal in lasso peptide biosynthesis based on penultimate isoleucine residue.

Lasso peptides are ribosomally synthesized peptides that undergo post-translational modifications including leader peptide removal by B (or the segregated B1 and B2) proteins and core peptide macrolactamization by C proteins to form a unique lariat topology. A conserved threonine residue at the penultimate position of leader peptide is hitherto found in lasso peptide precursors and shown to be a critical recognition element for effective enzymatic processing. We identified a lasso peptide biosynthetic gene cluster () from FA350, a Gram-negative and facultatively prey-dependent bacterium that belongs to a novel bacterial order in the class .

gen. nov., sp. nov., isolated from coastal sediment.

A Gram-stain-negative, facultative anaerobic, motile, rod-shaped and orange bacterium, designated A06, was obtained off the coast of Weihai, PR China. Cells were 0.4-0.

Niche Modification by Sulfate-Reducing Bacteria Drives Microbial Community Assembly in Anoxic Marine Sediments.

Sulfate-reducing bacteria (SRB) are essential functional microbial taxa for degrading organic matter (OM) in anoxic marine environments. However, there are little experimental data regarding how SRB regulates microbial communities. Here, we applied a top-down microbial community management approach by inhibiting SRB to elucidate their contributions to the microbial community during OM degradation.

sp. nov. and sp. nov., isolated from marine sediment.

Two Gram-stain-negative, strictly aerobic, chemoheterotrophic, short-rod-shaped and non-motile strains, forming yellow colonies and designated F47161 and F64183, were isolated from marine sediment of Xiaoshi Island, Wei Hai, PR China. Strain F47161 grew at 15-37 °C (optimally at 30 °C) and pH 6.0-9.

Predation capacity of Bradymonabacteria, a recently discovered group in the order Bradymonadales, isolated from marine sediments.

Bacterial predation is a vital feeding behavior that affects community structure and maintains biodiversity. However, predatory bacterial species in coastal sediments are comparatively poorly described. In this study, the predation capacity of all nine culturable Bradymonabacteria strains belonging to the recently discovered order Bradymonadales was determined against different types of prey.

Robiginitalea marina sp. nov., isolated from coastal sediment.

A Gram-stain-negative, orange, aerobic, non-motile, rod-shaped marine bacterium, designated as 2V75, was isolated from the coastal sediment of Xiaoshi Island, Weihai, China. The strain 2V75 grew at 20-45 °C (optimum, 37 °C), from pH 7.0 to 9.

Psychroserpens luteolus sp. nov., isolated from Gelidium, reclassification of Ichthyenterobacterium magnum as Psychroserpens magnus comb. nov., Flavihalobacter algicola as Psychroserpens algicola comb. nov., Arcticiflavibacter luteus as Psychroserpens luteus comb. nov.

A Gram-stain-negative, motile by gliding, catalase positive, facultative anaerobic strain, designated strain XSD401, was isolated from an unidentified Gelidium species of Xiaoshi Island, Shandong Province, China. The 16S rRNA gene sequence comparisons indicated strain XSD401 had a sequence similarity of 96.9% with Psychroserpens damuponensis KCTC 23539 and 96.

Description of Lujinxingia vulgaris sp. nov., isolated from coastal sediment via prey-traps.

Two Gram-stain negative, facultative anaerobic, oxidase-negative, catalase-positive bacilli, designated as strains TMQ4 and TMQ2, were isolated from Xiaoshi Island, China, using prey-traps. Growth was observed within the ranges 25-45 °C (optimally at 37 °C), pH 6.5-9.

Inducible expression of agar-degrading genes in a marine bacterium Catenovulum maritimus Q1 and characterization of a β-agarase.

Agar-degrading bacteria are crucial drivers for the carbon cycle in the marine environments due to their ability that use algae as a carbon source. Although numerous agar-degrading bacteria and agarases have been reported, little is known about expression levels of agar-degrading genes in wild strains. Here, the genome of an agar-hydrolyzing marine bacterium, Catenovulum maritimus Q1, was sequenced and annotated with 11 agarase and 2 neoagarooligosaccharide hydrolase genes.

Bradymonabacteria, a novel bacterial predator group with versatile survival strategies in saline environments.

Background: Bacterial predation is an important selective force in microbial community structure and dynamics. However, only a limited number of predatory bacteria have been reported, and their predatory strategies and evolutionary adaptations remain elusive. We recently isolated a novel group of bacterial predators, Bradymonabacteria, representative of the novel order Bradymonadales in δ-Proteobacteria.

gen. nov., sp. nov., a novel representative of the phylum and proposal of fam. nov., ord. nov. and class. nov.

is widespread and ecologically important in various anoxic environments. However, the portion of culturable bacteria within this phylum is quite low and, in fact, there is only one currently described species. In this study, a novel anaerobic, non-motile, coccoid, Gram-stain-negative bacterial strain, designated S-5007, was isolated from surface marine sediment.

Strategies for culturing active/dormant marine microbes.

Microorganisms are ubiquitous in the ocean environment and they play key roles in marine ecosystem function and service. However, many of their functions and phenotypes remain unknown because indigenous marine bacteria are mostly difficult to culture. Although many novel techniques have brought previously uncultured microbes into laboratory culture, there are still many most-wanted or key players that need to be cultured from marine environments.

gen. nov., sp. nov., isolated from a marine solar saltern.

A Gram-stain-negative, non-motile, fine rod or short filament shaped, jacinth pigmented bacterium, designated strain WDS2C27, was isolated from a marine solar saltern in Wendeng, Weihai, PR China (37°31'5″ N, 122°1'47″ E). Growth of WDS2C27 occurred at 20-42 °C (optimum 37 °C) and pH 6.5-8.

sp. nov., a new member of the family isolated from the red alga .

A Gram-stain-negative, rod-shaped (0.2-0.3×1.

sp. nov., a flavobacterium isolated from surface seawater.

A taxonomic study was carried out on strain SH27, which was isolated from seawater collected around Xiaoshi Island, PR China. Cells of strain SH27 were Gram-stain-negative, non-motile, rod-shaped, orange-pigmented and grew at 15-37 °C (optimum, 28 °C), at pH 6.0-8.

sp. nov., a rhodopsin-containing bacterium from an Antarctic environment and emended description of the genus .

A Gram-stain-positive, facultatively anaerobic bacterium, strain JDX10, was isolated from a soil sample of Fildes Peninsula, Antarctica. Cells of the strain were irregular rod-shaped and non-motile. Cells grew at 4-40 °C (optimum, 28 °C), at pH 6.

sp. nov., isolated from a freshwater lake.

A Gram-stain-negative, non-motile, rod-shaped, oxidase-positive, red-pigmented bacterium, strain N3, was isolated from Fuxian lake, a freshwater lake in Yunnan Province, PR China. Strain N3 was facultatively anaerobic, heterotrophic and negative for catalase. Optimal growth occurred at 30 °C (range 4-45 °C), pH 7.

sp. nov., a novel helical bacterium isolated from a sea cucumber culture pond.

Strain F3212, Gram-stain-negative, aerobic, helical and motile bacterium, was isolated from the marine sediment collected in a sea cucumber culture pond located in Rongcheng, China. Strain F3212 grew optimally at pH 8.5, at 30 °C and in the presence of 3.