Publications by authors named "Da Cheng Zhang"
[Alteration of PTEN gene expression affects the migration of human airway smooth muscle cells in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
March 2011
Objective: To investigate the changes in human airway smooth muscle cell (HASMC) migration and related signaling pathway after interference with PTEN gene expression.
Method: HASMCs were infected with an adenovirus vector and RNA interference vector of human PTEN gene to establish the cell model with PTEN gene over-expression (Ad-GFP-PTEN-HASMC) and one with PTEN gene silencing (Ad-shPTEN-HASMC), using Ad-GFP-infected and a blank cells as the negative controls and LY294002 as the positive control. Fluorescence microscopy and flow cytometric analysis were used to evaluate the transfection efficiency, and Western blotting was performed to examine the expression of PTEN and the activation of AKT and ERK1/2 signal pathway.
Objective: To observe the effect of exogenous phophatase and tensin homolog deleted on chromosone 10 (PTEN) gene transfer via recombinant adenoviruses on the proliferation of human airway smooth muscle cells (HASMCs) in vitro and investigate the possible mechanisms.
Methods: With a recombinant adenovirus vector containing PTEN (Ad-PTEN) constructed using the pAdxsi system, PTEN gene was transiently transfected into HASMCs and the transfection efficiency was determined by fluorescence microscope. RT-PCR and Western blotting were performed to detect the expression of PTEN mRNA and protein in the infected cells.
[Microelements in potato and lily samples studied by laser-induced breakdown spectroscopy technology].
Guang Pu Xue Yu Guang Pu Fen Xi
May 2009
A LIBS setup was built in the Institute of Modern Physics. In our experiments, LIBS spectra produced by infrared radiation of Nd:YAG nanosecond laser with 100 and 150 mJ pulse energy, respectively, were measured by fiber optic spectrometer in the ranges of 230-430 nm and 430-1 080 nm with a delay time of 1.7 and gate width of 2 ms for potato and lily samples prepared by vacuum freeze-dried technique.
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- The SEM examination of Chinese Tuber samples showed similar spore features to T. pseudohimalayense and other related species, suggesting they may be variations within a single species.
- Different researchers highlighted variations in the peridium's surface among T. indicum, T. himalayense, T. sinense, and T. pseudohimalayense, leading to the conclusion that they are all part of the same species - T. indicum.
- The study reveals that within T. indicum in China, there are two distinct groups based on genetic sequences, indicating a complex geographical structure among these Tuber species.
Article Synopsis
- The study examined the phylogenetic relationships of Tuber pseudoexcavatum with other truffle species using DNA sequences from four specific genes.
- The analysis revealed two main subclades of black truffles: one containing Asian varieties and the Perigord black truffle, and another consisting of T. pseudoexcavatum and T. brumale, indicating an early divergence between them.
- A proposed common ancestor for these black truffles suggests a geographical origin between Europe and China, with migrations occurring in distinct routes, leading to the development of different species.