Differential effects of divalent manganese and magnesium on the kinase activity of leucine-rich repeat kinase 2 (LRRK2).

Various mutations in leucine-rich repeat kinase 2 (LRRK2) have been linked to susceptibility for both familial and idiopathic late-onset Parkinson's disease (PD). In this study, we have demonstrated that phosphorylation of MBP and LRRKtide by the LRRK2 G2019S mutant was activated by Mn(2+) in vitro. This enhanced G2019S kinase activity was due to the combination of an increase in kinase and a decrease in ATPase activity by Mn(2+).

Merging social networking environments and formal learning environments to support and facilitate interprofessional instruction.

This study describes the redesign of an interprofessional team development course for health science students. A theoretical model is hypothesized as a framework for the redesign process, consisting of two themes: 1) the increasing trend among post-secondary students to participate in social networking (e.g.

Cyclic loading of notched dental composite specimens.

Objective: The purpose of this study was to examine the fracture toughness (K(IC)) of three direct dental composites and one indirect dental composite subject to cyclic loading.

Methods: The composites were a micro-filled (Micronew, Bisco INC., Schaumburg, IL, USA), a hybrid (Renew, Bisco INC.

White matter abnormalities and dystonic motor disorder associated with mutations in the SLC16A2 gene.

Aim: Mutations in the SLC16A2 gene have been implicated in Allan-Herndon-Dudley syndrome (AHDS), an X-linked learning disability* syndrome associated with thyroid function test (TFT) abnormalities. Delayed myelination is a non-specific finding in individuals with learning disability whose genetic basis is often uncertain. The aim of this study was to describe neuroimaging findings and neurological features in males with SLC16A2 gene mutations.

Midline location of tumour is a risk factor for postoperative vomiting in children requiring posterior fossa tumour resection.

Children requiring posterior fossa brain tumour surgery form a unique and significant neuro-oncology population. Postoperative vomiting (POV) is a problem for these children. Also, severe vomiting that is refractory to treatment has been seen clinically in these children, but it is not well described in the literature.

Children's vomiting following posterior fossa surgery: A retrospective study.

Background: Nausea and vomiting is a problem for children after neurosurgery and those requiring posterior fossa procedures appear to have a high incidence. This clinical observation has not been quantified nor have risk factors unique to this group of children been elucidated.

Methods: A six year retrospective chart audit at two Canadian children's hospitals was conducted.

Reliability of the adhesive remnant index score system with different magnifications.

Objective: To test the hypothesis that adhesive remnant index (ARI) scores show no differences when examined under different magnifications.

Materials And Methods: The study included 80 upper human premolars. Stainless steel brackets were bonded to the specimens with Transbond XT light cure adhesive and Transbond Plus Self Etch Primer (3M Unitek, Monrovia, Calif, USA).

The use of collagen cross-linking agents to enhance dentin bond strength.

Unlabelled: Type I collagen is a major component of the hybrid layer, and improvement of its mechanical properties may be advantageous during bonding procedures.

Objective: To investigate the effect of three different cross-linking agents (Glutaraldehyde [GD], Grape seed extract [GSE], and Genipin [GE]) on the tensile bond strength (TBS) of resin-dentin bonds.

Materials And Methods: Sixty-four sound human molars were collected and their occlusal surfaces were ground flat to expose dentin.

Anthropometry as a predictor of high speed performance.

To assess anthropometry as a predictor of high-speed performance, subjects performed four seated knee- and hip-extension workouts with their left leg on an inertial exercise trainer (Impulse Technologies, Newnan GA). Workouts, done exclusively in either the tonic or phasic contractile mode, entailed two one-minute sets separated by a 90-second rest period and yielded three performance variables: peak force, average force and work. Subjects provided the following anthropometric data: height, weight, body mass index, as well as total, upper and lower left leg lengths.

A class of 2,4-bisanilinopyrimidine Aurora A inhibitors with unusually high selectivity against Aurora B.

The two major Aurora kinases carry out critical functions at distinct mitotic stages. Selective inhibitors of these kinases, as well as pan-Aurora inhibitors, show antitumor efficacy and are now under clinical investigation. However, the ATP-binding sites of Aurora A and Aurora B are virtually identical, and the structural basis for selective inhibition has therefore not been clear.

Multiplexed analysis of peptide antigen-specific antibodies.

Many experiments require the simultaneous measurement of more than one parameter at a time. These protocols describe a multiplex method that can be used to detect two or more analytes simultaneously in the same sample preparation. Our example detects antibodies directed against peptide antigens but is amenable to most typical EIA format configurations to allow measurement of alternative analytes.

Inhibition of p75 neurotrophin receptor attenuates isoflurane-mediated neuronal apoptosis in the neonatal central nervous system.

Background: Exposure to anesthetics during synaptogenesis results in apoptosis and subsequent cognitive dysfunction in adulthood. Probrain-derived neurotrophic factor (proBDNF) is involved in synaptogenesis and can induce neuronal apoptosis via p75 neurotrophic receptors (p75). proBDNF is cleaved into mature BDNF (mBDNF) by plasmin, a protease converted from plasminogen by tissue plasminogen activator (tPA) that is released with neuronal activity; mBDNF supports survival and stabilizes synapses through tropomyosin receptor kinase B.

Impact of methionine oxidation on the binding of human IgG1 to Fc Rn and Fc gamma receptors.

Methionine oxidation commonly occurs in the Fc fragment of therapeutic monoclonal antibodies; however, its impact on antibody function has not been addressed. Using surface plasmon resonance and cell binding assays, we examined the impact of methionine oxidation on the binding of two humanized IgG1 antibodies to Fc gamma receptors (Fc gammaR) and to the neonatal Fc receptor (Fc Rn). A panel of Fc gammaRs, including Fc gammaRI, Fc gammaRIIa-131H, Fc gammaRIIa-131R, Fc gammaRIIb/c, Fc gammaRIII ALF, Fc gammaRIII ALV, and Fc gammaRIIIb was evaluated.

Fatigue behaviour of dental composite materials.

Objective: The intent of the project was to evaluate the fatigue behavior of particle and fiber filled dental composites that are fabricated either directly or indirectly using a notched specimen with respect to the number of cycles until failure.

Methods: The materials were five dental composites, three normally cured in the oral cavity (Restolux, Renew and Filtek Supreme), direct processing, and two laboratory produced (BelleGlass, and Tescera), indirect processing. The specimens were 3mmx3mmx25mm bars with a 0.

Production of monoclonal antibodies by glycoengineered Pichia pastoris.

The growing antibody market and the pressure to improve productivity as well as reduce cost of production have fueled the development of alternative expression systems. The therapeutic function of many antibodies is influenced by N-linked glycosylation, which is affected by a combination of the expression host and culture conditions. This paper reports the generation of a glycoengineered Pichia pastoris strain capable of producing more than 1 g l(-1) of a functional monoclonal antibody in a robust, scalable and portable cultivation process with uniform N-linked glycans of the type Man(5)GlcNAc(2).

Development and factor analysis of the Coaching and Athletic Training Questionnaire.

To explore athletes' perceptions related to coaching and athletic training care, the Coaching and Athletic Training Questionnaire was developed. A 10-item version was administered to 708 varsity athletes from three Division I universities in the National Collegiate Athletic Association. 341 women and 344 men completed questionnaires.

Molecular analysis of the beta-catenin gene in patients with the Mayer-Rokitansky-Küster-Hauser syndrome.

Purpose: To study the beta-catenin gene in a group of Mayer-Rokitansky-Küster-Hauser patients.

Methods: Twelve patients with the Mayer-Rokitansky-Küster-Hauser syndrome were included in this study. DNA was extracted from peripheral blood and the region codifying beta-catenin GSK-3beta phosphorylation sites on exon 3 was amplified.

Prognostic models and risk scores: can we accurately predict postoperative nausea and vomiting in children after craniotomy?

Postoperative nausea and vomiting (PONV) is a problem for many children after craniotomy. Prognostic models and risk scores help identify who is at risk for an adverse event such as PONV to help guide clinical care. The purpose of this article is to assess whether an existing prognostic model or risk score can predict PONV in children after craniotomy.

Degradation, fatigue, and failure of resin dental composite materials.

The intent of this article is to review the numerous factors that affect the mechanical properties of particle- or fiber-filler-containing indirect dental resin composite materials. The focus will be on the effects of degradation due to aging in different media, mainly water and water and ethanol, cyclic loading, and mixed-mode loading on flexure strength and fracture toughness. Several selected papers will be examined in detail with respect to mixed and cyclic loading, and 3D tomography with multi-axial compression specimens.

A pentacyclic aurora kinase inhibitor (AKI-001) with high in vivo potency and oral bioavailability.

Aurora kinase inhibitors have attracted a great deal of interest as a new class of antimitotic agents. We report a novel class of Aurora inhibitors based on a pentacyclic scaffold. A prototype pentacyclic inhibitor 32 (AKI-001) derived from two early lead structures improves upon the best properties of each parent and compares favorably to a previously reported Aurora inhibitor, 39 (VX-680).

Analysis of the degradation of a model dental composite.

Unlabelled: Dental composites undergo material property changes during exposure to the oral environment and may release compounds of potential toxicity, such as bisphenol A. Degradation of dental composites was studied in a simplified overlayer model in which bisphenol A diglycidyl methacrylate (BisGMA) was covalently bound to a porous silicon oxide surface. It was hypothesized that the chemical structure of this overlayer would allow release of bisphenol A, BisGMA, and the decomposition products thereof, upon exposure to water for an extended period.

Multiaxial analysis of dental composite materials.

Dental composites are subjected to extreme chemical and mechanical conditions in the oral environment, contributing to the degradation and ultimate failure of the material in vivo. The objective of this study is to validate an alternative method of mechanically loading dental composite materials. Confined compression testing more closely represents the complex loading that dental restorations experience in the oral cavity.