[Characteristics of rat kidney cell lines transformed by the DNA of human adenovirus type 6].

The data on the transforming activity of intact DNA of human adenovirus type 6 and that fragmented by restrictases Bam H I (31.3% of the genome), Bgl 2 (9.3% of the genome), and Hind 3 (7.

[Analysis of cell lines obtained from tumors induced in Syrian hamsters by SA7 adenovirus and its DNA].

Four cell lines derived from tumors induced by adenovirus SA7, its DNA (intact and fragmented with restrictase Sal-1), as well as isolated C-Sal-1 fragment (19.5% of genome) containing oncogene were studied. All the cell lines had the phenotypic markers of tumor cells, similar tumorigenicity, and produced T- and S-antigens.

Analysis of sequences of simian adenovirus SA7 (C8) DNA in transformed rat cells and hamster tumour cells.

Different methods of molecular hybridization were used to study DNA sequences of the highly oncogenic simian adenovirus SA7 (C8) present in the genomes of two transformed rat cell lines and in cells from three hamster tumours induced by adenovirus SA7. The entire DNA or the left-hand terminal SalI C fragment (19.5% of the genome) were employed.

[Isolation of cell lines transformed by highly oncogenic simian adenovirus SA7 and nononcogenic human adenovirus type 6 and their DNAs].

Methods for generation of cell lines transformed by highly oncogenic simian adenovirus SA7, nononcogenic human adenovirus type 6 and their DNAs are described. WAG rat kidney cells were used for transformation. To produce 1 focus of transformation, 1.

[Isolation and translation of late mRNA coding for the structural proteins of simian adenovirus SA7].

A total preparation of cytoplasmic RNA was isolated from late stage-infected African green monkey kidney cells using phenol extraction procedure. The poly (A)-containing mRNA fraction was selected on oligo(dT)-cellulose columns. The resulting mRNA preparations were heterogenous in size and contained about 20--60% of SA7-derived sequences.

[Biological properties of large-plaque clone 230 of simian adenovirus SA7(C8)].

The clone KB-230 of simian adenovirus SA7(C8) is described differing from the reference SA7(C8) strain and clones KB-2 and MB-1 by the presence of additional recognition sites when treated with different endonucleases. The KB-230 clone differs antigenically in the neutralization test from the MB-1 clone. Some biological and molecular-biological properties of the KB-230 clone were studied.

[Viral sequences in the genomes of rat and hamster cells transformed by simian adenovirus SA7(C8) and its DNA].

Four cell lines transformed by simian adenovirus SA7 and its DNA were analysed by means of molecular hybridization. Content of viral DNA sequences in different lines varied from 10% (the left end) of the molecule to the entire genomes. Transcription of the D BglII fragment (coordinates 1.

Integration of foreign genome fragments into cells transformed or cotransformed with fragmented adenoviral DNA.

The integration of DNA of highly oncogenic simian adenovirus type 7 (SA7) and non-oncogenic human adenovirus type 6 (Ad6) into the genome of newborn rat kidney cells transformed by fragmented DNA preparations was studied using reassociation kinetics and spot hybridization. Transforming DNA was fragmented with the specific endonuclease SalI (SA7) and BglII (Ad6). In contrast to the cell transformation by intact viral DNA, transformation by fragmented DNA resulted in integration into the cellular genome of not only the lefthand fragment with the oncogene but also of other regions of the viral genome.

[Identification of a new antigenic variant of simian adenovirus (SV30N)].

A new variant of simian adenovirus SV30-N is described. The variant is antigenically close to SV30 virus in the neutralization test but has some antigenic relationship to SA7 virus. The properties of SV30-N virus were retained after 5 clonings by the plaque method, after passage and cloning of the virus treated with immune sera to SV30 or SA7.

The distribution of guanine-cytosine pairs in adenovirus DNAs.

The distribution of guanine-cytosine (GC) pairs in the DNA of the highly oncogenic simian adenovirus type 7 (SA7) and the non-oncogenic human adenovirus type 6 (Ad6) has been studied by thermal denaturation and CsC1 density-gradient centrifugation. The differential of the DNA thermal denaturation curves shows the presence of pronounced peaks which indicates uneven distribution of GC pairs along the DNA chains and the presence of regions with GC content from 30 to 74% in SA7 DNA and from 40 to 68% in Ad6 DNA. The DNA restriction fragments obtained by treatment with EcoRI, BamHI, SalI, BglII and HindIII were subjected to CsC1 density-gradient centrifugation.

[Analysis of the structural proteins of human and simian adenoviruses].

Disk electrophoresis in polyacrylamide gel was used to study the polypeptide composition of purified virions of simian adenoviruses SA7, SV20 (H), and SV 38 as well as human adenovirus type 6. The general electrophoretic pattern of separation of structural polypeptides of simian and human adenoviruses was shown to be similar; the role of a number of components in the virion was elucidated. All the simian adenoviruses under study were found to have the following characteristic sizes of polypeptide components: components V and VII (DNA-framework) 50-51 and 18-19 kilodaltons, respectively, polypeptide VI (prihexon component) 29-30 kilodaltons.

[Isolation of adeno-associated virus type 4 from a culture of green monkey kidney cells].

In the process of biological control of uninfected green monkey kidney (GMK) cell cultures a thermostable hemagglutinating agent designated No. 5056 was isolated alongside with adenovirus-SV. By its antigenic properties the 5056 strain was identified as adeno-associated virus type 4 (AAV-4).

[Experimental infection of green monkeys with adenoassociated virus].

Primary infection and reinfection with adeno-associated virus type 4 (AAV-4) was reproduced in green monkeys experimentally infected with AAV-4 in mixture with adenovirus. Wide dissemination of the satellite virus in animals was observed. AAV-4 and its antigen were detectable 5 to 23 days after inoculation.

[Physical characteristics of simian adenovirus SA7].

Physical characteristics of simian adenovirus SA7 virion were studied. The buoyant density of SA7 virions was found to be 1.356 g/cm3.

[Transforming activity and the oncogenicity of simian adenovirus SA7 DNA].

The transforming and oncogenous activity of uncleaved DNA of simian adenovirus SA7 (AdSA7) and the products of its restriction by endonucleases R. Bam HI and R. SalI was studied.

[Etiological study of the fatal cases in the period of the influenza A epidemic of 1977-1978].

The etiology of the disease, the age structure of fatalities, the time of death were studied in 34 fatal cases in the period of influenza A (H1N1) epidemic in December 1977-February 1978 and in 33 fatal cases during influenza A/Victoria/3/75 (H3N2) epidemic in January-March, 1975 (data from some autopsy offices of Kiev). The results of the investigation of the etiology by immunofluorescence and the histological methods were mostly similar. The polyetiological structure of the diseases in the influenza epidemic of 1975 and rather monoetiological one in the influenza epidemic of 1977-1978 were observed.

[Fragmentation of the DNA of simian adenovirus type 38 by using restriction endonucleases].

The effect of restricting endonucleases Eco R I, BgI II and Sal I on simian adenovirus type 38 (SV-38) DNA was studied. Bgl II restrictase cleaves the virus DNA into 4 fragments, A, B, G, and D, with molecular weights 9.3 x 10(6), 3.

Physicochemical properties and restriction maps of simian adenovirus type 38 DNA.

The sedimentation constant of simian virus type 38 (SV-38) DNA was estimated to be 31.6S. The intrinsic viscosity of DNA was on average 86.

[Physical characteristics of human adenovirus type 6].

The size, chemical composition and the buoyant density of the virion, sedimentation and diffusion constants of human adenovirus type 6 were determined. The molecular mass of the virion was calculated on the basis of the values of sedimentation and diffusion constants and electron microscopy data.

The spread of rhinoviruses in the Soviet Union (USSR) and in Czechoslovakia (CSSR) and the role of their pathology.

Virological, serological and immunofluorescence studies revealed circulation of rhinoviruses of the strains 1A, 1B, 2, 3, 7, 9, 10, 12, 13, 14, 16-23, 27, 29-33, 42, 48, 53, 56, 60 and 69 on the territory of Czechoslovakia and the Soviet Union. According to virological results, type 48 predominated and was followed in frequency of occurrence by types 27, 14 and 16 in the USSR and 30, 1A and 31 in the CSSR. RV infection in adults with ARD diseases was the aetiology in 28.

[Helper viruses of adeno-associated virus type 4 replication].

In replication of adeno-associated virus type 4 (AAV-4) the helper function may be performed by a non-defective virus from the same group of parvoviruses (Kilham virus). The synthesis of AAV-4 antigen was observed in a pig embryo kidney cell line, SPEV, chronically infected with Kilham virus, strain RV-13, 45--52 passages. A one-day-old SPEV-Kilham culture was infected with AAV-4.

Biophysical properties of virions of human adenovirus of the type 6 and its DNA.

A study has been made of human adenovirus type 6 (Ad 6) which belongs to the C subgroup of the nononcogenic adenoviruses. The buoyant density of the Ad 6 virions in CsCl gradient was 1.3545 +/- 0.