Annulate lamellae observed in human prostatic carcinoma.

Annulate lamellae (AL) were observed in only three out of 40 cases of human prostatic carcinoma, but not in 20 cases of benign prostatic hyperplasia and 11 cases of presumably normal prostatic tissues. AL showed the continuity with the rough endoplasmic reticulum and seemingly the nuclear membrane consisting of lamellar or concentric arrangement of stacked membranes and occasionally annular structures. In addition, annuli were detected in the rough endoplasmic reticulum near the stacked membranes which were devoid of ribosomal attachment.

Virus particles in normal prostate tissues of mice from ten strains, with special reference to type-B virus particles.

Prostate tissues of a total of 61 normal mice from 10 different strains, including high (C3H/Dm, RIII/Dm, and A/Dm) and low (BALB/c/Dm, C3Hf/Bi/Dm, and C3Hf/He/TEX) mammary cancer, and high (AKR/Dm) and low (CBA/J/Cr, SJL/J/Cr, and C57/BL/6/TEX) leukemia strains were examined by electron microscopy for the presence of virus particles. These studies demonstrated that type-B virus particles were present in normal prostate tissues of some old mice from all the three high (C3H/Dm, RIII/Dm, and A/Dm) and one low (BALB/c/Dm) mammary cancer strains. They further demonstrated that varying number of type-C virus particles were present in prostate tissues of some young and old mice, including those from all the 10 strains, especially in a larger number in older mice, and that intracisternal type-A virus particles were observed in all the mice examined.

Histology and ultrastructure of the R-3327 C-F transplantable prostate tumor of Cophenhagen-Fisher rats.

The R-3327 C-F tumor is an adenocarcinoma of the prostate maintained by serial transplantation in male Copenhagen-Fisher hybrid rats. The tumor is histologically different from other tumor sublines derived from the original R-3327 tumor discovered in 1961 in an aged Copenhagen rat. Light and electron microscopy have shown that the R-3327 C-F tumor contains a well organized glandular epithelium with myoepithelial cells and a continuous basement membrane, although epithelial cells were much less differentiated than epithelial cells of either dorsal or lateral normal prostate of the rat.

Electron microscopic studies of intracisternal virus particles in Soehner-Dmochowski murine sarcoma virus-induced bone tumors of New Zealand Black rats.

Soehner-Dmochowski murine sarcoma virus (Moloney)-induced bone tumors of New Zealand Black rats carry two morphologically different types of virus particles, namely, extracellular type C and intracisternal virus particles, which have thus far not been reported. These two types of virus particles have also been observed in the tissue culture cells derived from normal prostate tissues of A/Dm and BALB/c/Dm mice after inoculation of cell-free extracts of these bone tumors. The intracisternal virus particles, 90 to 120 nm in diameter, have always been found in the rough endoplasmic reticulum; they have two inner concentric layers with a relatively electron-lucent center, frequently showing cylindrical, chain-like, or multipolar budding forms.

In situ embedding method of cells grown in beem capsules for immunoelectron microscopic studies of oncornaviruses.

A technique of in situ embedding of cells grown in BEEM capsules has been devised for immunoelectron microscopic studies of oncornaviruses. As compared to other immunoelectron microscopic procedures, this technique is less time and reagent-consuming. The quality and specificity of this method were tested on well-characterized mouse mammary tumor virus (type B virus) and murine sarcoma virus (type C virus particles).

Particle--lamella complexes in a case of human benign prostate hyperplasia: brief communication.

Particle--lamella complexes (PLC's), described for the first time, were found in glandular epithelial cells of the hyperplastic prostate tissues from a patient with transitional cell carcinoma of the urinary bladder. PLC's observed in this patient were similar to those seen in human hematopoietic neoplastic cells. They showed cylindroid forms and were composed of concentrically arranged lamellae and particles found in rows between these lamellae.

"Particle-filament complex" in tumor cells of northern pike, Esox lucius L.

Examination of the cells of lymphoreticular neoplasms of the northern pike (Esox lucius L.) by electron microscopy has demonstrated the presence of unusual cytoplasmic crystalloid inclusions in cells of a spontaneous trunk tumor. The ultra-structural analysis revealed that the inclusions are composed of parallel arrays of filaments associated with rows of particles.

Immunoelectron microscopic studies of antibodies in mouse sera directed against mouse mammary tumor virus.

Indirect immunoferritin and fixed immunofluorescence tests were carried out on (a) sera of mice hyperimmunized with isologous mouse mammary tumor virus (MMTV) particles or isologous MMTV-producing mammary tumor cells grown in tissue culture and (b) sera of mammary tumor-bearing and tumor-free mice of several inbred strains. Sera were tested against MMTV produced by C3H/HEJ/Tex tissue culture cells (MMT-1). Mammary tumor-bearing A/Dm, C3H/HeTex, and RIII/Dm mice and apparently tumor-free A/Dm mice were found to develop naturally occurring nonprotective anti-MMTV antibodies, whereas sera of tumor-free C3H/HeTex, RIII/Dm, and C57BL/6/Tex female mice and A/Dm, C3H/HeTex, and RIII/Dm male mice did not contain anti-MMTV antibodies.

Virus-like particles in a case of human prostate carcinoma.

Two morphologically different types of intracisternal virus-like particles were observed electron microscopically in a biopsy specimen of human prostate cancer. Particles of one type were 150-200 nm in diameter and contained either an electron-dense core or two concentric inner layers. Particles of the other type were smaller, 80-100 nm in diameter, and appeared mostly in filamentous or chainlike formation.

Search for oncogenic viruses in human prostate cancer.

Electron microscopic, immunologic, and biochemical methods have been used in an attempt to detect and characterize oncornaviruses in human prostatic carcinoma (PCa) and benign prostatic hyperplasia (BPH), and in prostates of mice of high and low mammary cancer or leukemia strains. Ultrastructural examination of 37 PCa and nine BPH specimens has revealed the presence of particles resembling type C virus in five cases of PCa and one of BPH, and also two different types of intracisternal virus-like particles in seven other cases of PCa. Type B virus particles have been observed in prostate of old mice of high mammary cancer strains, while type C virus particles have been found in the prostates of most mice of all the ten strains examined.

Transforming factors in human sarcoma cells in tissue culture.

Experimental data are presented which demomstrate the existence of a transmissible factor(s) in the majority of samples of leukemic bone marrow. This factor(s) is associated with the presence of a cytoplasmic antigen which can be detected by fixed immunofluorescence test with sera of patients with osteosarcoma, leukemia and some apparently normal individuals. Cultures of leukemic bone marrow carrying this factor(s) also form multinucleated cells when exposed to RD114 virus or cels.

Viral type A and type B hepatitis: morphology, biology, immunology and epidemiology--a review.

Viral hepatitis is one of the most serious infectious diseases in the United States and is of great concern to the public health agencies, hospitals and research laboratories. Progress in our knowledge of this disease has been based on cooperation between specialists in many diverse scientific disciplines employing sophisticated scientific instruments and technics. Close cooperation between clinical pathologists and clinicians is of great importance in diagnosis.

Ultrastructural studies of human prostatic neoplasia.

Forty-two specimens of human prostatic neoplasia (32 carcinomas, eight benign hyperplasia, two bladder tumors infiltrating prostatic tissue, and 15 tissue cultures derived from prostatic neoplasia) were examined by electron microscopy. Intracisternal viruslike particles, 150-200 nm in diameter and budding, were found in epithelial cells of four carcinomas. In some of these particles, an electron-dense central core or two concentric layers were discernible.

Characterization of an established cell line (SH-3) derived from pleural effusion of patient with breast cancer.

Spontaneous emergence of fast-growing cells in prolonged culture of pleural effusion cells obtained from a patient with breast cancer has led to the establishment of a cell line designated as SH-3. By morphological criteria, as revealed by light and electron microscopy, SH-3 cells are epithelial and resumble the poorly differentiated cells of a series of established human tumor cell lines. Their karyotype is hypotetraploid and different from that of HeLa cells.

Implications of humoral antibody in mice and humans to breast tumor and mouse mammary tumor virus-associated antigens.

As a part of a program directed toward the elucidation of the role of viruses in mouse and human breast cancer, a variety of immunological techniques were applied to a study of the humoral immune response of mice and of humans to their breast tumors. Tumor-bearing mice were found to produce antibodies against a complex array of tumor cell-associated antigens, including mouse mammary tumor virus (MMTV), components, heterophile and Forssman-like antigens, embryonic antigens, and possibly other tumor-associated antigens. Mice bearing MMTV-positive tumors had high titer antibodies against both viral and heterophile antigens.

Morphological, biological, immunological and biochemical studies on bone tumors of animals and man.

Biological studies on FBJ osteosarcoma virus in tissue cultures have led to the isolation of murine sarcoma virus. Characteristic type C-MuLV particles were observed in bone tumors induced by the SD-MSV-M-virus in vitro and in vivo. The SD-MSV-M virus also induced bone tumors in rats of all strains tested, and it has a similar tumor-inducing property in hamsters.