[Methodical approaches to evaluation of air pollution by emissions of motor vehicles in population areas].

There are results of comparative analysis of air pollution by emissions of motor vehicles in the residential districts of Ryazan via different methodical approaches. Emissions were calculated regarding analysis of the traffic intensity on the elements of the city traffic network. Relative emissions, equivalent relative emissions and relative coefficient of emission hazard were calculated for each district.

[The CT-guided transthoracic biopsy of lung's and mediastinal tumors].

The results of 107 CT-guided transthoracic biopsies, conducted during 2005-2010 yy, were analyzed. The authors have shown the advantages of the core biopsy compared with the fine-needle aspiration biopsy (FNAB). Sensitivity of the first for the malignant tumors was 93%, while the FNAB sensitivity was only 75%.

[Changes of motor-evacuatory function of the gastrointestinal tract after surgery in children with acute appendicitis].

A new method of computed phonoenterography (CPEG) evaluates objectively motor-evacuatory function of the gastrointestinal tract. Comparative study of changes in motor-evacuatory function of the intestine was carried out according to CPEG data in 60 children aged from 6 to 15 years after traditional surgery with Volkovich-Dyakonov incision, and after endovideosurgical appendectomy for plegmonous and gangrenous appendicitis. Inflammation of the appendix leads to inhibition of intestinal motor function.

[Evaluation of the efficiency of use of the bed fund of a central district hospital].

The paper deals with a complex sociohygienic study of the basic indices of resource provision of the public health system in the Tver Region and with an analysis of the scope of inpatient care to the population of the Torzhok District, Tver Region. Based on an in-depth examination, the efficiency of the use of the bed fund of the central district hospital (CDH) was evaluated and economic losses from inadequate use of beds and economic effects of work of daily CDH-based hospitals were calculated.

[Working conditions and morbidity of workers at the present-day shoe manufacturing factory].

Poor working factors of present-day industry (SALAMANDER technology) include high intensity of labour, monotonicity, forced posture, constant wide-range noise, fumes of organic solvents, dust of organic origin, etc. The working conditions in producing departments are considered to be of degrees of 1-3 of hazard class 3. The highest incidence rates were noted in age groups under 30 years and above 50 years and in workers with over 10-year length of service.

[Optimization of a method for purifying antibodies for immunofluorescent diagnosis of influenza].

Efficiency and diagnostic adequacy of immunoglobulin isolation from rabbit immune serum by reiterated salting out with ammonium sulfate and subsequent separation of DEAE sephadex A-50 was evaluated. Electrophoresis and immunoelectrophoresis in agar gel demonstrated that the resultant immunoglobulin fraction is much more pure than after one salting out. It contains nothing more than antibodies to influenza virus taken for immunization, and after binding to the stain shows high fluorescent activity and induces but a moderate background nonspecific fluorescence in the subsequent preparations.

[Gastrointestinal motility disorders in late stages of adhesive intestinal obstruction in children].

The results of examination and treatment of 211 patients with late adhesive ileus (LTAI) are discussed. Highly sensitive noninvasive method of phonoenterography was used for LTAI diagnosis and control of the efficacy of non-operative treatment. The gastrointestinal motor-evacuation disorders were studied in a group of patients with chronic recurring LTAI.

[Extracellular alkaline ribonucleases produced by Bacilli strains from the permafrost of the Kolyma lowland].

Aerobic spore-forming bacteria were isolated from the permafrost of the Kolyma lowland. Two strains of bacilli are shown to produce a relatively large amount of extracellular low-molecular weight alkaline RNases. The N-terminal amino acid sequences of the RNases secreted by these strains are similar.

[Primary structure and catalytic properties of extracellular ribonuclease from Bacillus circulans].

A comparative research of individual peptide structures obtained after hydrolysing of Bacillus circulans and B. amyloliquefaciens RNases by the Glu-specific staphylococcal protease was carried out by means of mass-spectrometry and Edman degradation methods. A complete amino acid sequence of B.

[Complete primary structure of Bacillus thuringiensis extracellular ribonuclease].

Complete primary structure of an extracellular low molecular mass ribonuclease of Bacillus thuringiensis was determined using Edman degradation and mass-spectrometry analysis of individual peptides obtained after hydrolysis of the protein by cyanogen bromide and staphylococcal protease. The peptides were isolated and purified by HPLC and denaturing PAGE. The enzyme consists of 109 amino acid residues (Asp 8, Asn 6, Thr 6, Ser 10, Glu 3, Gln 1, Pro 3, Gly 9, Ala 12, Val 7, Ile 7, Leu 7, Tyr 7, Phe 4, His 1, Arg 10, Trp 3 and Lys 5) and has a molecular weight of 12182 Da.

[Two forms of extracellular low molecular weight Bacillus sp. BCF 247 ribonuclease. Isolation and characteristics of the protein].

Two homogeneous samples of low molecular mass RNAase (RNAases Bci I and Bci II) were obtained from cultural filtrates of spore-forming bacteria strain Bacillus sp. BCF 247 isolated from permafrost soils. The yields of RNAases Bci I and Bci II were 17% and 16% at the 17388- and 15376-fold degree of purification, respectively.

[Extracellular alkaline ribonuclease of Bacillus thuringiensis var. subtoxicus].

Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B.

[Extracellular ribonuclease from Bacillus pumilus].

The extracellular ribonuclease (RNAse Bp) was isolated from the cultural medium filtrate of Bacillus pumilus by ammonium sulfate precipitation and two stages of ion-exchange chromatography on carboxymethyl- and phospho-cellulose columns. The amino acid composition and N-terminal amino acid residue have been determined. The kinetic parameters of cleavage reaction of synthetic polynucleotides have been measured.

[Isolation and characteristics of intracellular nuclease isoforms from Serratia marcescens].

Two enzyme forms were isolated from the commercial preparation of extracellular endonuclease of Serratia marcescens strain B10 M1. The chromatographic and electrophoretic properties, isoelectric points and N-terminal amino acid residues are different for both enzymes. At the final step of the purification procedure including ion-exchange chromatography on phospho- and DEAE-cellulose columns the yields of nucleases Sm1 and Sm2 were 13% and 25%, respectively.

[Isolation of isoforms and crystallization of endonucleases of Serratia marcescens].

Two isoforms of an extracellular endonuclease, nuclease Sm1 and nuclease Sm2, were isolated from the culture filtrate of Serratia marcescens strain B10 M1 by the ligand-exchange chromatography on iminodiacetate-agarose in Cu2(+)-form, and chromatography on phosphocellulose and DEAE-Toyopearl 650S. The pI for nucleases Sm1 and Sm2 were found to be 7.1 and 6.

[Amino acid sequence of ribonuclease Ap1 from Aspergillus pallidus].

The complete amino acid sequence of an extracellular guanyl-specific RNase from Aspergillus pallidus fungi has been established. The RNase contains 104 amino acid residues (Mr 11,029). Its primary structure was analyzed basing on the automated Edman degradation of the carboxymethylated RNase followed by tryptic digestion and sequencing of the resultant hydrolysate.

[Extracellular guanyl-specific ribonuclease Sa from the actinomycete Streptomyces aureofaciens. Primary structure and homology with ribonucleases from bacteria and fungi].

The complete amino acid sequence of a guanyl-specific RNAse from Streptomyces aureofaciens has been established using a rapid method of primary structure analysis which eliminates the peptide fractionation. The automated Edman degradation of the carboxymethylated RNAse Sa and of non-fractionated peptide mixtures produced by tryptic and staphylococcal protease digests of the modified protein were used. The RNAse contains 96 amino acid residues, Mr 10,566.