The eosinophilic variant of acute myelomonocytic leukemia developing as a secondary leukemia in a patient with mycosis fungoides.

The eosinophilic variant of acute myelomonocytic leukemia (AMML) is highly associated with characteristic abnormalities of chromosome 16. Leukemias developing secondary to chemotherapy frequently show abnormalities of chromosomes 5 and 7. This report describes a secondary AMML, eosinophilic variant (M4Eo), developing in a patient with coexistent mycosis fungoides who had received chemotherapy, including an alkylating agent, and showing characteristic abnormalities of both chromosomes 7 and 16.

Nutritive value of poultry by-product meal. 1. Metabolizable energy values as influenced by method of determination and level of substitution.

Experiments were conducted to determine the metabolizable energy (MEn) of eight poultry by-product meal (PBPM) samples. Samples averaged 5.44% moisture, 4.

Inhibitory neurones of a motor pattern generator in Xenopus revealed by antibodies to glycine.

Glycine and gamma-aminobutyric acid (GABA) are inhibitory transmitters of major importance. Whereas neurones using GABA as the transmitter can be visualized by immunocytochemical methods for glutamate decarboxylase (GAD) or GABA, no comparable techniques have been available for the selective visualization of glycinergic neurones. We have now produced polyclonal antibodies which specifically recognize glycine in glutaraldehyde-fixed tissue.

Excitatory synaptic drive for swimming mediated by amino acid receptors in the lamprey.

In order to investigate the properties and pharmacology of the excitatory synaptic drive received by motoneurons during swimming in the lamprey, propriospinal excitatory interneurons were activated as a population by the regional application of N-methyl-D,L-aspartate (NMA) to either the 6-8 rostral-most or the 6-8 caudal-most segments of lengths of isolated spinal cord. This caused a rhythmic motor output to be generated in these regions. Synaptic potentials that were phase-locked to, and dependent on, the rhythmic motor activity of the segments exposed to the agonist could be recorded in motoneurons lying outside the activated regions.

Dual-component synaptic potentials in the lamprey mediated by excitatory amino acid receptors.

The synaptic mechanisms underlying amino acid-mediated excitation in the lamprey spinal cord have been investigated. Fine stimulating electrodes were used to stimulate single axons in the spinal cord and evoke unitary EPSPs in lamprey motoneurons and one type of premotor interneuron, the CC interneuron. Three types of EPSP, distinguished by their time course and sensitivity to amino acid antagonists, were seen.

Acute myelomonocytic leukemia (M-4 subtype) with abnormal marrow eosinophilia and a normal chromosome 16.

A variety of chromosome 16 abnormalities, including inversion, deletion, and translocation in patients with acute myelomonocytic leukemia and abnormal marrow eosinophilia have been reported recently. The authors have identified an AMML patient who had a normal karyotype in 50 metaphases. In particular, chromosome 16 was closely examined for abnormality and was found to be entirely normal.

Synaptic potentials in motoneurons during fictive swimming in spinal Xenopus embryos.

Embryos spinalized at the 3rd to 6th postotic myotome and immobilized in 10(-4) M tubocurarine can respond to a brief skin stimulus with motor root activity suitable for swimming. Embryos spinalized at the more caudal levels give shorter episodes of fictive swimming. We have previously described the synaptic inputs to motoneurons during fictive swimming in intact embryos (23).

Reciprocal inhibitory interneurones in the Xenopus embryo spinal cord.

The mechanism of reciprocal inhibition between antagonistic motor centres during swimming in the paralysed Xenopus embryo has been investigated further. Paired intracellular recordings have been made from interneurones and motoneurones in an attempt to identify neurones which make direct inhibitory synapses onto motoneurones on the opposite side of the spinal cord. A physiological class of inhibitory interneurones is described which, when stimulated by intracellular current passage, evoke short-latency, probably monosynaptic, strychnine-sensitive inhibitory potentials in contralateral motoneurones.

Dual-component amino-acid-mediated synaptic potentials: excitatory drive for swimming in Xenopus embryos.

The neuronal basis of the excitation received by motoneurones during swimming in curarized Xenopus embryos has been investigated further. Extracellular stimulation of axons in the fibre tracts of the spinal cord has been used to evoke unitary excitatory post-synaptic potentials (p.s.

Correlation of protein content of feedstuffs with the magnitude of nitrogen correction in true metabolizable energy determinations.

The relationship between the protein content of feedstuffs and the magnitude of difference between corresponding true metabolizable energy (TME) and nitrogen-corrected TME ( TMEn ) was investigated. Fifty-two samples of feed ingredients, mixtures of ingredients, and mixed feeds were assayed for TME and TMEn . A positive relationship was observed between the protein content of the feed samples and the magnitude of difference between determined TME and TMEn values.

Excitatory amino acid receptors in Xenopus embryo spinal cord and their role in the activation of swimming.

Bath application of N-methyl-D-aspartate (NMDA), kainate or quisqualate to Xenopus embryos depolarized spinal cord motoneurones and reduced their input resistance in both normal salines and salines containing 20 mM-Mn2+ and 0.5 mM-Ca2+, or 2 X 10(-6) M-tetrodotoxin. This suggests that motoneurones possess all three types of excitatory amino acid receptor.

Comparison of heat production of chickens measured by energy balance and by gaseous exchange.

An open-circuit respiration calorimeter suitable for chickens or other small animals has been constructed. The system contains two animal chambers and is capable of determining heat production of two animals or groups of animals independently and simultaneously with gas collection apparatus for continuous sampling of chamber gases. The operation of the system was tested in two experiments by determining heat production of two groups of 10-week-old, full-fed cockerels over a 4-day period both by energy retention (body balance) and gaseous exchange.

True metabolizable energy of fats at low level dietary inclusion.

When assayed at a 2.5% level of inclusion in a corn-soybean meal basal diet with 40 replications per treatment, no significant differences could be demonstrated between the true metabolizable energies (TME) of corn oil and two samples of tallow varying in stearic acid (18:0) content. All values were in excess of the gross energy of fat, suggesting an improvement in the absorption of other dietary constituents.

Additive toxicity of aflatoxin and dietary tannins in broiler chicks.

In two experiments graded levels of either aflatoxin and tannic acid (experiment 1) or aflatoxin and a high-tannin variety of grain sorghum (BR-64) (experiment 2) were incorporated into broiler chick starting diets in a factorial design. When present singly, aflatoxin and both tannic acid and high tannin sorghum significantly depressed chick growth. When present in combination, and deleterious effects of aflatoxin and either tannic acid or high tannin sorghum on chick growth and feed conversion ratios were found to be additive.

Additivity of true metabolizable energy values as measured with roosters, broiler chicks and poults.

True metabolizable energy (TME) values were determined for five feed ingredients (yellow corn, dehulled soybean meal, corn gluten meal, menhaden fish meal, and poultry byproduct meal), and three combinations of these ingredients, using Single Comb White Leghorn roosters, 6-week-old broiler males (Cobb), and 6-week-old male turkey poults (Large White). There was generally good agreement in th TME values between roosters, broilers, and poults although the values for broilers tended to be slightly lower than for roosters or poults. Values for the ingredient combinations as assayed with the three classes of birds showed differences of 3% or less between "predicted" and "determined" in seven of nine comparisons, indicating a satisfactory degree of additivity of TME values for these ingredients.

Effect of diet composition on feed intake and growth of chicks under heat stress. II. Constant vs. cycling temperatures.

Broiler chicks 5 to 7 weeks of age were subjected to a constant cool (14 +/- 1 C) or hot (31 +/- 1 tc) environment and fed diets varying in fat level and energy and nutrient density. Chicks gained significantly (P less than or equal to .01) more body weight in the cool than in the hot environment.

Calcium metabolism after renal transplantation.

Calcium metabolism was studied in four patients who had undergone renal transplantation from eight to 19 months previously. The studies included conventional biochemical and radiological parameters, undecalcified bone histology and metabolic balances. The only abnormality found was biochemical and histologic evidence of mild hyperparathyroidism.