Spindle coma: incidence, clinicopathologic correlates, and prognostic value.

Three hundred seventy comatose patients were studied clinically and electrographically. Of these, 5.7% showed the "spindle coma" electroencephalogram (EEG) pattern.

Serum lysozyme levels in inflammatory bowel disease.

Serum lysozyme levels were compared in patients with Crohn's disease (CD) and chronic ulcerative colitis (CUC) to determine if the two diseases could be differentiated by this parameter. The lysozyme concentrations were measured by three different methods: 1. the turbidimetric clearance of Micrococcus lysodeikticus utilizing egg white lysozyme as a standard; 2.

System for simplified discontinuous-gradient polyacrylamide-gel electrophoresis.

We describe a modified polyacrylamide gel electrophoresis method with which plasma proteins are excellently resolved in 4 h. We made a three-layer discontinuous gradient gel with concentrations of 80,60, and 45 g of polyacrylamide per liter of tris(hydroxymethyl)aminomethane/ethylenediaminetetraacetic acid/borate buffer, pH 9.2.

The frequency of precipitins to trichloroacetic acid-extractable antigens from thermophilic actinomycetes in farmer's lung patients and asymptomatic farmers.

The hypothesis that the trichloroacetic acid (TCA)-extractable antigen fractions prepared from Micropolyspora faeni cells and other thermophilic actinomycetes are better diagnostic immunodiffusion reagents for the detection of farmer's lung disease than the crude extracellular antigen concentrates has been tested. The frequency of serum precipitins to the TCA-extractable antigens has been compared in symptomatic farmer's lung patients and in healthy farmers having positive precipitin tests to the crude antigen concentrates of the analogous thermophilic actinomycetes. Sixty-eight per cent of the symptomatic group were found to have precipitins to the M.