CO-Based micro-respirometry for measuring bacterial load under aerobic and anaerobic conditions.

The bacterial load (BL), or total viable count, of aerobes can be measured using micro-respirometry, %O-μR, in which the consumption of dissolved O is monitored with respect to incubation time, . In %O-μR the 'bioreactor' often comprises a canonical plastic tube with a small %O sensor; it is simple, fast and accurate and used in automated, commercial instruments for measuring BL. Here we show that it is also possible to measure BL using a new form of micro-respirometry, %CO-μR, in which the production of CO in the growth medium is monitored.

Early wound infection monitoring via headspace O micro-respirometry.

A luminescence based, inexpensive, 3D printed O indicator is incorporated into a commercial, clear, occlusive wound dressing, which allows the %O in the headspace above a simulated wound to be monitored. Two wound models are used to evaluate this micro-respirometry-based system for monitoring wound infection namely, a simple 'agar plug' model and a wounded porcine skin model. Inoculation of either wound model with E.

Colorimetric CO Indicators.

Carbon dioxide, CO, is an essential part of life, in that through green plant photosynthesis it is used to generate food and fuel and is generated in both aerobic and anaerobic respiration. Industrially, it is used in fire extinguishers, supercritical fluid extractions, and food packaging. Environmentally, it is in the atmosphere, hydrosphere, and biosphere and is responsible for global warming and the acidification of the oceans.

Ethnicity and sex-specific 99th percentile upper reference limits of high-sensitivity cardiac troponin I among adults in Xinjiang, China.

Objectives: To determine the 99th percentile upper reference limit (URL) of high-sensitivity cardiac troponin I (hs-cTnI) in a healthy population in Xinjiang, China, and investigate the impact of ethnicity, sex, and age on this limit.

Design And Methods: From September 2018 to March 2022, 5,090 Han and Uyghur adults aged 20-79 years were recruited. After questionnaire screening, 2,970 participants with physical and/or laboratory normality were enrolled.

Non-invasive, 3D printed, colourimetric, early wound-infection indicator.

A simple, non-invasive, colour-based carbon dioxide (CO) indicator is described. The indicator provides an indirect response to the rapid, aerobic microbial colonisation of an underlying wound when used in conjunction with an occlusive ( sealed) dressing. The indicator has potential as an early warning indicator of infection in chronic wounds.