[Luteolin inhibits proliferation of lung cancer A549 cells by increasing ROS production and inhibiting the AKT/mTOR signaling pathway and HO-1 expression].

Objectives: To investigate the mechanism of luteolin for inhibiting proliferation of lung cancer A549 cells.

Methods: A549 cells treated with different concentrations of luteolin for 48 h were evaluated for changes in cell viability, proliferation, reactive oxygen species (ROS) production and apoptosis using MTT assay, plate cloning assay, EdU staining, DCFH-DA assay and Hoechst33258 staining. The changes in cell autophagy were examined with MDC staining, and the expressions of apoptosis-related proteins (Bax, Bcl-2, and cleaved caspase-9), autophagy-related proteins (LC3B, Beclin 1, and P62), AKT/mTOR pathway proteins, and HO-1 protein were detected using Western blotting.

Osteosarcoma-Targeting Pt Prodrug Amphiphile for Enhanced Chemo-Immunotherapy via Ca trapping.

Platinum (Pt)-based anticancer agents exhibit a lack of selectivity in the treatment of osteosarcoma, resulting in significant toxicity. Furthermore, immune surveillance withinthe tumor microenvironment impedes the uptake of platinum drugs by osteosarcoma cells. To overcome these challenges, an oxaliplatin-based Pt prodrug amphiphile (Lipo-OXA-ALN) was designed and synthesized by incorporatingan osteosarcoma-targeting alendronate (ALN) alongside a lipid tail.

Photoinduced electron transfer enables cytochrome P450 enzyme-catalyzed reaction cycling.

Cytochrome P450 enzymes (CYPs), the members of the largest superfamily of enzymes in plant kingdom, catalyze a variety of functional group transformations involved in metabolite biosynthesis, end-product derivatization, and exogeneous molecule detoxification. Nevertheless, CYPs' functional characterization and practically industrial application have been largely encumbered by their critical dependency on the reducing equivalent for the catalytic cycling, driven by the tedious electron relay mediated by CYP reductase (CPR). Here, we report a photoinduced electron transfer system that initiates and sustains the CYP-catalyzed reaction cycling.

Microalgal-bacterial biofilms enhance pollutant removal coupling with eicosapentaenoic acid production in high-concentration ammonia‑nitrogen wastewater.

Microalgal-bacterial biofilms have emerged as a promising approach for wastewater treatment. However, its potential to treat high-concentration ammonia‑nitrogen wastewater coupling with high-value fatty acid production remains unclear. Therefore, this study explored the efficiency of a microalgal-bacterial biofilm in treating high-concentration ammonia‑nitrogen wastewater and its ability to produce high-value fatty acids, with the activated sludge (bacteria) and microalgal-bacterial suspension as control.

Laser processing materials for photo-to-thermal applications.

Photothermal conversion materials (PCMs) are crucial component in solar-thermal energy technologies. Although various PCMs with excellent sunlight harvesting have been developed for colorful solar-thermal applications, uniform and large-scale production of PCMs remains a challenge, and the PCMs prepared through the conventional methods are often non-site specific. Laser processing technology (LPT), as an efficient, convenient, green and sustainable technology, can directly create micro/nano structures and patterns at specific locations on materials surface, attracting widespread attention in photo-to-thermal applications.