Partial purification and properties of pectin lyase from Penicillium expansum.

A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.

Physiological and energetic aspects of bacterial metabolite overproduction.

This review attempts to provide a rational explanation for microbial metabolite over-production, a phenomenon manifest with many wild-type and mutant organisms. After analysing the relationships between catabolic and anabolic processes within the growing cell (which point to the presence of sufficient kinetic control elements to ensure a stringent coupling between the two), consideration is given to mechanisms that might allow the rate of catabolism to be varied independently of the rate of anabolism. It is in this latter context that an excretion of specific metabolites appears to play a key role.

Quantitative aspects of glucose metabolism by Escherichia coli B/r, grown in the presence of pyrroloquinoline quinone.

Escherichia coli B/r was grown in chemostat cultures under various limitations with glucose as carbon source. Since E. coli only synthesized the glucose dehydrogenase (GDH) apo-enzyme and not the appropriate cofactor, pyrroloquinoline quinone (PQQ), no gluconate production could be observed.