Isotope-assisted metabolic flux analysis as an equality-constrained nonlinear program for improved scalability and robustness.

Stable isotope-assisted metabolic flux analysis (MFA) is a powerful method to estimate carbon flow and partitioning in metabolic networks. At its core, MFA is a parameter estimation problem wherein the fluxes and metabolite pool sizes are model parameters that are estimated, via optimization, to account for measurements of steady-state or isotopically-nonstationary isotope labeling patterns. As MFA problems advance in scale, they require efficient computational methods for fast and robust convergence.

Effect of Light Regiment on Farrowing Performance and Behavior in Sows.

The purpose of this study was to investigate the effects of light exposure on farrowing performance in sows. Thirty sows were moved to the farrowing unit at d 110 of gestation and assigned a treatment: 12 h light/12 h dark cycle (Dark) or 24 h light (Light). Treatments began upon entry into the farrowing unit.

Liposome-based measurement of light-driven chloride transport kinetics of halorhodopsin.

We report a simple and direct fluorimetric vesicle-based method for measuring the transport rate of the light-driven ions pumps as specifically applied to the chloride pump, halorhodopsin, from Natronomonas pharaonis (pHR). Previous measurements were cell-based and methods to determine average single channel permeability challenging. We used a water-in-oil emulsion method for directional pHR reconstitution into two different types of vesicles: lipid vesicles and asymmetric lipid-block copolymer vesicles.

NetRed, an algorithm to reduce genome-scale metabolic networks and facilitate the analysis of flux predictions.

Flux balance analysis (FBA) of large, genome-scale stoichiometric models (GSMs) is a powerful and popular method to predict cell-wide metabolic activity. FBA typically generates a flux vector containing O(1,000) fluxes. The interpretation of such a flux vector is difficult, even for expert users, because of the large size and complex topology of the underlying metabolic network.

Effects of increased levels of supplemental vitamins during the summer in a commercial artificial insemination boar stud.

Heat stress due to increasing extremes in ambient temperature and humidity results in reduced semen quality in boars. This has caused reduced efficiency of the swine industry, requiring more boars to breed the same number of sows. Vitamins such as vitamin C (VC) and E (VE) have been shown to improve semen quality in boars.