Direct and indirect effects of multiplex genome editing of F5H and FAD2 in oil crop camelina.

Mutants with simultaneous germline mutations were obtained in all three F5H genes and all three FAD2 genes (one to eleven mutated alleles) in order to improve the feed value of the seed meal and the fatty acid composition of the seed oil. In mutants with multiple mutated F5H alleles, sinapine in seed meal was reduced by up to 100%, accompanied by a sharp reduction in the S-monolignol content of lignin without causing lodging or stem break. A lower S-lignin monomer content in stems can contribute to improved stem degradability allowing new uses of stems.

Two new terpenoids from the leaves and twigs of Croton cnidophyllus and their anti-AD activities.

A new oleanane-type triterpenoid, 3β-acetyl-15α-hydroxy-oleanane-13β,28-olide (1), and a new clerodane furanoditerpenoid, cnidophyllin A (2), together with eleven known compounds (3-13) were isolated and identified from the 95% EtOH extract of the leaves and twigs of Croton cnidophyllus. Except for compounds 3 and 7, all other compounds were isolated for the first time from C. cnidophyllus.

NVP-2, in combination with Orlistat, represents a promising therapeutic strategy for acute myeloid leukemia.

Cell cycle dysregulation and the corresponding metabolic reprogramming play significant roles in tumor development and progression. CDK9, a kinase that regulates gene transcription and cell cycle, also induces oncogene transcription and abnormal cell cycle in AML cells. The function of CDK9 for gene regulation in AML cells requires further exploration.

Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response.

Sulforaphane (SF) is a sulfur (S)-containing isothiocyanate found in cruciferous vegetables and is known for its potent anticancer properties. Broccoli sprouts, in particular, are considered safe and healthy dietary choices due to their high SF content and other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) is an excellent approach to enhance the abundance of SF.

Optimization of Naringin Extraction, Synthesis of Dihydrochalcone and Its Effects on Reducing Blood Lipid Levels In Vitro.

Response surface methodology (RSM) was used to optimize the extraction process of naringin. The central component design included three parameters of extraction, namely temperature (X), solid-liquid ratio (X), and extraction time (X). The optimum extraction temperature was 67 °C; the ratio of feed to solvent was 54:1 mL/g, and the extraction time was 2.