Publications by authors named "D Verner-Jeffreys"
Article Synopsis
- The study aimed to establish epidemiological cut-off values for the minimum inhibitory concentrations (MICs) of 10 antimicrobial agents against the bacteria Vibrio parahaemolyticus using standardized testing methods.
- Eight laboratories conducted broth microdilution tests at two different temperatures (35°C and 28°C) to assess the effect of incubation temperature on MIC values, finding that many values were consistent across both temperatures.
- The generated data will be submitted to the Clinical and Laboratory Standards Institute to help set international standards for interpreting antimicrobial susceptibility tests for this species.
The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°°C and read after 44-48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration (MIC) and disc diffusion were derived from data obtained by 1 laboratory and 2 laboratories respectively. The present work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-off values from aggregations of these data with previously published data.
View Article and Find Full Text PDFOne Health is a recognition of the shared environment inhabited by humans, animals and plants, and the impact of their interactions on the health of all organisms. The COVID-19 pandemic highlighted the need for a framework of pathogen surveillance in a tractable One Health paradigm to allow timely detection and response to threats to human and animal health. We present case studies centered around the recent global approach to tackle antimicrobial resistance and the current interest in wastewater testing, with the concept of "one sample many analyses" to be further explored as the most appropriate means of initiating this endeavor.
View Article and Find Full Text PDFIn order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h.
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