Screening of Plant UDP-Glycosyltransferases for Betanin Production in Yeast.

To cover the rising demand for natural food dyes, new sources and production methods are needed. Microbial fermentation of nature-identical colours, such as the red pigment betanin, has the potential to be a cost-efficient alternative to plant extraction. The last step of betanin production is catalysed by a UDP-glycosyltransferase (UGT).

INCAWrapper: a Python wrapper for INCA for seamless data import, -export, and -processing.

Motivation: INCA is a powerful tool for metabolic flux analysis, however, import and export of data and results can be tedious and limit the use of INCA in automated workflows.

Results: The INCAWrapper enables the use of INCA purely through Python, which allows the use of INCA in common data science workflows.

Availability And Implementation: The INCAWrapper is implemented in Python and can be found at https://github.

Regioselective Glycosylation of Polyphenols by Family 1 Glycosyltransferases: Experiments and Simulations.

Family 1 glycosyltransferases (GT1s, UGTs) form natural product glycosides with exquisite control over regio- and stereoselectivity, representing attractive biotechnological targets. However, regioselectivity cannot be predicted and large-scale activity assessment efforts of UGTs are commonly performed via mass spectrometry or indirect assays that are blind to regioselectivity. Here, we present a large high performance liquid chromatography screening discriminating between regioisomeric products of 40 diverse UGTs (28.

Sequence mining yields 18 phloretin C-glycosyltransferases from plants for the efficient biocatalytic synthesis of nothofagin and phloretin-di-C-glycoside.

C-glycosyltransferases (C-GTs) offer selective and efficient synthesis of natural product C-glycosides under mild reaction conditions. In contrast, the chemical synthesis of these C-glycosides is challenging and environmentally harmful. The rare occurrence of C-glycosylated compounds in Nature, despite their stability, suggests that their biosynthetic enzymes, C-GTs, might be scarce.