[Hypermethylation of the human calcitonin gene as a molecular marker in acute lymphoid leukemia].

HpaII/MspI blot-hybridization analysis of the 5'-end region of the calcitonin (CT) gene methylation in cells of bone marrow and peripheral blood of patients with acute lymphoblastic leukemias (ALL) has been carried out. ALLs are accompanied by hypermethylation of the inner cytosine in the CCGG sequences of this region of the CT gene. The level of hypermethylation of the CT gene corresponded to the degree of disease progression and malignancy.

[The lack of the CpNpG methylation at the 5'-terminal region of the human calcitonin gene in norm and in leukemia [].

The inner cytosine methylation was analyzed in the CCWGG sequences of the 5'-terminal region of the human calcitonin gene from peripheral blood and bone marrow cells in various forms of leukemia. Since these sequences remain nonmethylated both in norm and in various leukemia forms, the CpG dinucleotide hypermethylation of the 5'-terminus of the human calcitonin gene, characteristic for the development of leukemias, does not spread over adjacent CpNpG sequences.

[Changes in the functional activity of human neutrophils under the action of recombinant interleukin-2].

The effect of recombinant interleukin 2 (rIL-2) on the functional activity of human polymorphonuclear cells (neutrophils) was studied. The study revealed that the preliminary incubation of neutrophils with rIL-2 at a concentration of 10 I.U.