[Identification of a new bioregulator acting in ultralow doses in bulb onion (Allium cepa L.)].

A bioregulator that has physicochemical and biological properties similar to a group of bioregulators isolated from various animal tissues has been found in the bulb onion (Allium cepa L.). It was determined that the biological action of the plant bioregulator is determined by a peptide with molecular weight of 4036 +/- 2 Da whose 18-C-terminal amino acid sequence consisted of 18 residues.

[Study of a new group of bioregulators isolated from the greater plantain (Plantago major L.)].

Proteins with physicochemical properties and biological activity similar to those of membrano-tropic homeostatic tissue-specific bioregulators that had been found earlier in various animal tissues were discovered in leaves of the common plantain (Plantago major L.). To study the specific activity of these plant proteins, we developed an experimental model for organotypic roller cultivation of newt (Pleurodeles waltl) skin tissue in vitro.

[Modulators of the regulatory protein activity acting at microdoses].

New, previously not studied bioregulators active in the ultra low doses corresponding of 10(-8) - 10(-17) mg/ml have been isolated from vitreoretinal tissue of eye. It has been shown that these bioregulators comprise some regulatory peptides-modulators represented by proteins with molecular weights 15-70 KDa one of which is bovine serum albumin. Correlation between the nanosize of bioregulators and their ability to show activity in ultra low doses is established.

[Effect of adhesion on the status of corneal tissue during in vitro culturing].

Newt and rat cornea with and without the corneal limbus was cultured under conditions of adhesion to a substrate (stationary culturing) and in the absence of adhesion (roller culturing). It was found that the contact of the tissue with the substrate play the key role in the transduction of the regulatory signal that ensures the maintenance of cornea viability and affects proliferation and migration of cornea cells.

[Role of a regulatory protein from the bovine cornea in activation of cell sources of corneal regeneration in vitro].

A highly purified regulatory protein isolated from the bovine cornea (RBC) was tested for the effect on the rat and newt corneas in vitro under different culture conditions. In the newt cornea, RBC stimulated limbus epithelial cells in roller cultures and cells in the basal layer of corneal epithelium in both roller and stationary cultures. In roller cultures of the rat cornea, RBC had no effect on weakly differentiated limbus cells but stimulated progenitor cells of the basal cornea layer to differentiation inbto definitive epithelial cells.