[Dermal mast cells density examination as an aspect of mechanical injuries vitality diagnostics].

Objective: To examine the changes in dermal mast cells density in the mechanically injured skin and to evaluate the applicability of dermal mast cells density increase as an injuries vitality diagnostic criteria (serial examination, statistically processed results).

Material And Methods: 240 skin autopsy samples with mechanical injuries from 40 persons were divided to 3 groups (80 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with mast cells tryptase antibodies was performed consequented with histological slides full-format scanning and computer processing.

[Detection of some markers of the early phase of the inflammatory and reparative process in mechanical skin injuries].

Objective: To examine the applicability of IHC staining method: with TGF-β1 antibodies (serial examination, statistically processed results) and with mast cell tryptase antibodies for injuries vitality determination.

Material And Methods: 261 skin autopsy samples with mechanical injuries from 29 persons were divided to 3 groups (87 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC both with TGF-β1 and mast cells tryptase antibodies was performed.

[TGFβ1 antibodies application for IHC determination of injuries vitality. The experimental results].

Is to examine the applicability of IHC staining method with TGFβ1 antibodies for injuries vitality determination. 72 skin autopsy samples with mechanical injuries from 12 persons were divided to 3 groups (=24 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with TGFβ1 antibodies was performed.

Dynamics of γ-aminobutyric acid concentration in the human brain in response to short visual stimulation.

Objective: To find a possible quantitative relation between activation-induced fast (< 10 s) changes in the γ-aminobutyric acid (GABA) level and the amplitude of a blood oxygen level-dependent contrast (BOLD) response (according to magnetic resonance spectroscopy [MRS] and functional magnetic resonance imaging [fMRI]).

Materials And Methods: fMRI data and MEGA-PRESS magnetic resonance spectra [echo time (TE)/repetition time (TR) = 68 ms/1500 ms] of an activated area in the visual cortex of 33 subjects were acquired using a 3 T MR scanner. Stimulation was performed by presenting an image of a flickering checkerboard for 3 s, repeated with an interval of 13.

[Forensic pathological diagnostics of rare T-cell leukemia in re-examination].

A forensic pathological case of rare T-cell leukemia is reported. Autopsy samples (paraffine blocks and slides) from 32 years old female with long-term existing anemia and leukopenia, number of chronic inflammatory diseases, died from fulminant pneumonia, were presented for forensic pathological re-examination. A routine histological examination was done using standard H&E stain.