A method is suggested to isolate and purify glycosaminoglycans forming elements of a joint from different kinds of connective tissue. The suggested method has been used to study the amount and composition of glucosaminoglycans in the knee joint tissues of people who had died from different accidents. The cartilage of patella and medial condyle of the femur, synovial membrane, medial meniscus and medial tendon of musculus quadriceps femoris are studied.
View Article and Find Full Text PDFA new method for the isolation of the urine glycosaminoglycans (GAG) with the use of ethanol is suggested. Experimental conditions for ethanol sedimentation and purification of GAG have been selected. It is suggested that the urine GAGs be divided into sulfated and nonsulfated with the use of resochin and their uronic acids and hexoses be examined.
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