[Generation of transgenic animals, expressing alpha- and beta-chains of autoreactive TCR].

Transgenic animal studies has become a key approach for gene function analysis as well as for modeling of different human diseases, including autoimmune diseases caused by activation of T-lymphocyte clones whose TCRs possesses high affinity for syngeneic MHC molecules. In this study we cloned genes, encoding alpha- and beta- chains of autoreactive TCR of hybridoma 7, specific for syngeneic MHC class II molecules A(b). Amplified DNA fragments, containing rearranged genomic DNA of alpha- and beta-chains of hybridoma 7 were cloned into special cassette vectors, containing natural promoter and enhancer elements for direct expression of genes encoding TCR alpha- and beta-chains in T-lymphocytes of transgenic animals.

[Coreceptor function of CD4 in response to MHC class I molecule].

Specificity of T cell receptor (TCR) and its interaction with coreceptor molecules play decisive role in successful passing of T lymphocytes via check-points during their development and finally determine the efficiency of adaptive immunity. Genes encoding alpha- and beta-chains of TCR hybridoma 1D1 have been cloned. The hybridoma 1D1 was established by the fusion of BWZ.

[Endovascular correction of a multi-level arterial lesion in an elderly patient with lower-limb critical ischaemia].

Presented herein is a clinical case report showing possibilities of endovascular surgery in comprehensive treatment of a multi-level stenosing lesion in an elderly male patient presenting with pyo-necrotic alterations of the crus and foot. A 67-year-old male patient suffering from diabetes mellitus, lower-limb vessels atherosclerosis, critical ischaemia, indolent wound of the left foot following amputation of the toes was subjected to collaterally approached balloon-mediated angioplasty of critical stenoses of the left common iliac artery and deep femoral artery. During the operation, we used elements of coronary interventions technique.

Suppression of primary allogenic response by CD8+ memory cells.

Immune response to allogenic tumor cells is associated with the appearance of long-living CD8+ memory cells capable of rapid restimulation and lysis of tumor cells in case of repeated injection of these cells. In order to acquire the effector function, allorestricted memory cells need antigen restimulation for 2 days, which is a specific feature of central memory cell population. These cells can suppress proliferation of naive splenocytes in vitro.