Quantitative and morphometric data indicate precise cellular interactions between serotonin terminals and postsynaptic targets in rat substantia nigra.

We have quantified the density of serotonin axonal varicosities, their synaptic incidence and their distribution among potential targets in the pars reticulata and pars compacta of the rat substantia nigra. Serotonin axonal varicosities, counted at the light microscopic level following in vitro [3H]serotonin uptake and autoradiography, amounted to 9 x 10(6)/mm3 in the pars reticulata and 6 x 10(6)/mm3 in the pars compacta, among the densest serotonin innervations in brain. As determined at the electron microscopic level following immunolabelling for serotonin, virtually all serotonin varicosities in the pars reticulata and 50% of those in the pars compacta formed a synapse, essentially with dendrites.

Acetylcholine innervation of sensory and motor neocortical areas in adult cat: a choline acetyltransferase immunohistochemical study.

Light microscopic choline acetyltransferase (ChAT) immunocytochemistry was used to examine the distribution of the acetylcholine innervation in primary motor (4 gamma) and sensory (3a, 3b, 41 and 17) cortical areas of adult cat. In every area, scattered immuno-reactive cell bodies were present and a relatively dense meshwork of ChAT immunoreactive axons pervaded the whole cortical thickness. These axons were generally thin and bore innumerable varicosities of different sizes.

Ultrastructural characterization of the acetylcholine innervation in adult rat neostriatum.

The ultrastructural features of acetylcholine axon terminals (varicosities) in adult rat neostriatum were characterized by electron microscopy after immunostaining with a sensitive monoclonal antibody against rat choline acetyltransferase. Several hundred single sections from these varicosities were analysed for shape, size and content, presence of a synaptic membrane specialization, and composition of the microenvironment. An equivalent number of unlabeled varicosities selected at random from the same micrographs were similarly examined.

Decrease and long-term recovery of choline acetyltransferase immunoreactivity in adult cat somatosensory cortex after peripheral nerve transections.

The functional reorganization of cerebral cortex following peripheral deafferentation is associated with changes in a number of neurotransmitters and related molecules. Acetylcholine (ACh) enhances neuronal responsiveness and could play a role in activity-dependent cortical plasticity. In this study, choline acetyltransferase (ChAT) immunohistochemistry was used to investigate ACh innervation of the primary somatosensory cortex in cats sustaining complete unilateral forearm and paw denervations.

Relational features of acetylcholine, noradrenaline, serotonin and GABA axon terminals in the stratum radiatum of adult rat hippocampus (CA1).

In a well-defined sector of adult rat hippocampus (CA1, stratum radiatum), the ultrastructural features of acetylcholine (ACh), noradrenaline (NA), serotonin (5-HT) and GABA axon terminals (varicosities) were compared by electron microscopy after immunostaining with antibodies against choline acetyltransferase, NA, 5-HT and glutamic acid decarboxylase. Approximately 100 sectional profiles of each type were analyzed for size, presence of a synaptic membrane specialization (synaptic incidence) and composition of the microenvironment. An equivalent number of immunonegative varicosity profiles selected at random from the same micrographs were similarly examined.