Publications by authors named "D Tomkova"
Article Synopsis
- Chromatin controls gene expression through epigenetic modifications, particularly focusing on histone H3, specifically lysine 27 (H3K27), which is modified by the Polycomb Repressive Complex 2 (PRC2) to regulate developmental genes.
- Research using a non-modifiable version of H3K27 in Arabidopsis revealed severe developmental issues similar to PRC2 mutants, such as early flowering and increased callus formation.
- The study uncovers new roles for H3K27 in determining plant cell functions and metabolic processes, indicating its significant impact on stem elongation and lignin composition.
The maintenance of genetic information is important in eukaryotes notably through mechanisms occurring at the nuclear periphery where inner nuclear membrane proteins and nuclear pore-associated components are key factors regulating the DNA damage response (DDR). However, this aspect of DDR regulation is still poorly documented in plants. We addressed here how genomic stability is impaired in the () double mutants showing defective nuclear shaping.
View Article and Find Full Text PDFAn ongoing challenge in functional epigenomics is to develop tools for precise manipulation of epigenetic marks. These tools would allow moving from correlation-based to causal-based findings, a necessary step to reach conclusions on mechanistic principles. In this review, we describe and discuss the advantages and limits of tools and technologies developed to impact epigenetic marks, and which could be employed to study their direct effect on nuclear and chromatin structure, on transcription, and their further genuine role in plant cell fate and development.
View Article and Find Full Text PDFBackground And Purpose: Neuronal protein S100B assays are available now with a perspective of being an early screening tool for serious intracranial injury. The aim of the study was to correlate early S100B measurements and initial CCT findings in the patients sustaining mild traumatic brain injury (MTBI).
Methods: The prospective study included patients of all ages with a history of MTBI.
Objective: To find out severity of brain injury in alcohol intoxicated patients after mild (GCS 13-15) brain injury.
Methods: A retrospective study of all patients older than 15 years in whom cranial CT (CCT) scan was performed between October 2006 and September 2007. Information of alcohol intoxication, injury mechanism, details of facial/head injuries, admission GCS, CCT findings and type of treatment were retrieved from patients' medical records.