A rapid and easy PCR-RFLP method for genotyping Serratia marcescens strains isolated in different hospital outbreaks and patient environments in the Lyon area, France.

A new genotyping method for Serratia marcescens is described. This method uses the flagellin gene as target for polymerase chain reaction amplification and Alu I restriction fragment length polymorphism. The strains tested belonged to 13 different hospital clusters of S.

[The value of parameters of iron metabolism in the differential diagnosis of anemias].

As a result of investigations into the diagnostic validity of selected haematologic-morphological and clinical-chemical test factors of iron metabolism in the diagnosis of hypochromic anaemia, the examined test faktora are differently evaluated as individual parameters and in their combination. 1. Haematocrit (PCV) is equal to the determination of haemoglobin concentration as a search parameter.

[Optimizing the diagnosis of anemia].

The specific value of the erythrocyte parameters MCH and MCV for the differential-diagnostic arrangement of anaemias to the use of the haematological automations of analysis PHA 1/2 is represented. A prerequisite is the precise determination of haemoglobin concentration and erythrocyte number. MCHC has no sufficient discriminatory capacity.

Release of Fc-receptors after streptococcal lysis induced by a lytic enzyme from Streptomyces globisporus.

Streptomyces globisporus produced an enzyme which had lytic effects on streptococci and was able to solubilize their Fc-receptors. A new method for rapid purification of this enzyme was described. It consisted of adsorption to Amberlite CG 50 and subsequent chromatography on CM-cellulose Whatman CM 52.