Quantitative Biodistribution of OMVs Using SPECT/CT Imaging with HYNIC-Duramycin Radiolabeling.

Bacterial outer membrane vesicles (OMVs) are emerging as important players in the host-microbiome interaction, while also proving to be a promising platform for vaccine development and targeted drug delivery. The available methods for measuring their biodistribution, however, are limited. We aimed to establish a high-efficiency radiolabeling method for the treatment of OMVs.

Cardiovascular autonomic and peripheral sensory neuropathy in women with obesity.

Introduction: A higher incidence of neural dysfunction in people with obesity has been described. We determined the prevalence of neuropathic lesions in obese women and evaluated their potential association with anthropometric and laboratory parameters.

Patients And Methods: In our cross-sectional study, we enrolled female patients with obesity and without diabetes before obesity treatment.

Evaluation of NO and PN Emission in Relation to Actuator Control.

This study aimed to investigate the interrelationships between key harmful emission components, nitrogen oxides (NO), and particulate numbers (PNs) in diesel engine exhaust and the control actuators of diesel engines. This research involved conducting a series of experiments under fixed parameters within an engine brake laboratory environment to elucidate these correlations. The objectives of this study were to conduct a comprehensive review of the relevant emissions technology literature and a comparative assessment of particle measurement methods based on dilution ratios and develop innovative aerosol preparation principles tailored to condensation particle measurement.

Radiolabeling of Platelets with Tc-HYNIC-Duramycin for In Vivo Imaging Studies.

Following the in vivo biodistribution of platelets can contribute to a better understanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the platelets with a radioisotope. In this study, we report a new method to label platelets with Tc, the most frequently used isotope for SPECT in clinical applications.

Probing binding and occlusion of substrate in the human creatine transporter-1 by computation and mutagenesis.

In chordates, energy buffering is achieved in part through phosphocreatine, which requires cellular uptake of creatine by the membrane-embedded creatine transporter (CRT1/SLC6A8). Mutations in human slc6a8 lead to creatine transporter deficiency syndrome, for which there is only limited treatment. Here, we used a combined homology modeling, molecular dynamics, and experimental approach to generate a structural model of CRT1.