Laser speckle contrast imaging with principal component and entropy analysis: a novel approach for depth-independent blood flow assessment.

Current study presents an advanced method for improving the visualization of subsurface blood vessels using laser speckle contrast imaging (LSCI), enhanced through principal component analysis (PCA) filtering. By combining LSCI and laser speckle entropy imaging with PCA filtering, the method effectively separates static and dynamic components of the speckle signal, significantly improving the accuracy of blood flow assessments, even in the presence of static scattering layers located above and below the vessel. Experiments conducted on optical phantoms, with the vessel depths ranging from 0.