[Contribution of L,D-carboxypeptidases in virulence of facultative intracellular pathogenic bacteria Listeria monocytogenes].

Aim: Evaluate influence of mutation of Listeria monocytogenes genes coding murein-tetrapeptide L,D-carboxypeptidase Lmo0028 and Lmo1638 on dynamics of infectious process and interaction of purified muropeptides with NOD1 receptor.

Materials And Methods: Wild type EGDe strain and recombinant strains GIMins1638 H GIMins0028 obtained on its basis by site-specific mutagenesis were used. Infectious process dynamics was studied on the model of intravenous infection of BALB/c mice.

[Influence of mutation L,D-carboxypeptidase-coding gene on dynamics of Listeria infection].

Aim: To study the progress of infection caused by mutant Listeria monocytogenes with deletion of Imo0028 gene coding L,D-carboxypeptidase (protein involved in metabolism of peptidoglycan).

Materials And Methods: Deletion of Imo0028 gene was performed with site-specific mutagenesis method using wild-type strain EGDe. Virulence was studied on mice of BALB/c line.

[Identification of proteins determining the virulence of Listeria monocytogenes using the bioinformation analysis].

The postgenomic stage of biotechnology allows the bioinformation approaches to be used for revealing previously unknown factors involved in different processes. In this work, bioinformation approaches were applied to analysis of factors involved in the L. monocytogenes virulence.