Sub-second characterization of locomotor activities of mouse models of Parkinsonism.

The degeneration of midbrain dopamine (DA) neurons disrupts the neural control of natural behavior, such as walking, posture, and gait in Parkinson's disease. While some aspects of motor symptoms can be managed by dopamine replacement therapies, others respond poorly. Recent advancements in machine learning-based technologies offer opportunities for unbiased segmentation and quantification of natural behavior in both healthy and diseased states.

Open-source platform for kinematic analysis of mouse forelimb movement.

Here we present an open-source behavioral platform and software solution for studying fine motor skills in mice performing reach-to-grasp task. We describe steps for assembling the box, training mice to perform the task, and processing the video with the custom software pipeline to analyze forepaw kinematics. The behavioral platform uses readily available and 3D-printed components and was designed to be affordable and universally reproducible.

Oxidative Status in Different Areas of the Cerebral Cortex of Wistar Rats during Focal Ischemia and Its Modulation with Carnosine.

Oxidative status was assessed in different areas of the cerebral cortex of male Wistar rats under normal condition and during permanent 24-h focal ischemia. In intact animals, the level of lipid hydroperoxides in the frontal lobes of both hemispheres was by 36% higher than in other cortical areas, while total antioxidant activity was by 25% higher than in other areas. During ischemia, changes in oxidative status were localized only in the ischemic focus and penumbra zone and did not involve other cortical areas.

[Imprinting as a mechanism of information memorizing in the adult BALB/c mice].

Study of spatial learning in adult BALB/c mice revealed that a short exposition to the environment (from 3 to 8 minutes) could be enough for spatial information to be fixed in the long-term memory, and affected subsequent learning process in the new environment. Control group, learning in the same maze, followed the "shortest path" principle during formation of the optimal food-obtaining habit. Experimental animals, learning in a slightly changed environment, were unable to apply this rule due to persistent coupling of the new spatial information with the old memory traces which led to constant errors.