Spectral and biological characteristics of some Acyclovir salts.

In the present work there are described some spectral characteristics in the ultraviolet and infrared region under various environment conditions for a series of synthetic analogues of deoxyguanosine, substances possessing antiviral properties. The study is performed on the Acyclovir compounds and on its Na, K and Li salts, synthetized in Romania, in comparison with the similar product Zovirax, of the "Wellcome" firm (England), used in the clinical practice for several years. The results show a very marked resemblance of the spectral behavior for all these products, a conclusion confirmed by the similar biological effects in the herpetic infection.

Testing of the in vivo therapeutic efficacy on the cutaneous herpes simplex infections of the autochthonous product 5% Acyclovir ointment.

The experimental results of the in vivo testing of an autochthonous pharmaceutical Acyclovir form prepared for the topical treatment of herpetic infections with a mucocutaneous location are shown in this paper. This testing on laboratory animals continues the in vivo performed investigations regarding the antiviral activity of this compound, which have proved that the efficacy of the inhibitory action exerted by the product on the Herpes simplex virus multiplication is comparable with the characteristics of the standard substance (Acyclovir-Zovirax Wellcome). By testing the therapeutic efficacy of the autochthonous Acyclovir preparation on an experimental model of cutaneous herpes infection in the newborn rat, it is demonstrated in a statistically significant manner that the product exerts a strong inhibitory action on the virus multiplication at the level of epidermis (proved by the lowering of virus production in the cutaneous tissue); the result is a drastic reduction of local herpes vesicles and of virus propagation in the neuraxis attended by the appearance of herpes meningo-encephalitis with a lethal course.

Testing of some pharmacological characteristics of the autochthonous product Acyclovir.

In the present work, the preliminary results of a study regarding the pharmacokinetic properties of the autochthonous synthetic drug Acyclovir are shown. The in vitro and in vivo toxicologic test demonstrates a low toxicity of this product, the drug being well tolerated both by the laboratory animal and by the fibroblasts of the human embryo. The specific antiviral action of the product on the multiplication in vitro of the Herpes simplex virus types 1 and 2, appreciated by the lowering capacity of the infection titre and by the determination of the inhibitory concentration 50% (IC50), is significant and comparable with previous results obtained by the authors by testing the drug Zovirax of the Welcome firm.

[The action of amphiphilic drugs on human fibroblasts in vitro. Their possible use in antiviral and antitumor chemotherapy].

The LDH cytotoxicity test is able to emphasize the stabilisation of human embryo fibroblast membranes, in vitro, by two amphiphilic drugs: the metomidate and the thymolol. The membrane stabilisation is emphasized by the reduced LDH externalization as compared with the untreated cells. The use of the LDH test for the selection of some natural complexes or synthetic drugs with membrane stabilising and potential antiviral activity is proposed.