Publications by authors named "D Richmond"
Background & Aims: Validated scoring methods such as the Mayo Clinic Endoscopic Subscore (MCES) evaluate ulcerative colitis (UC) severity at the worst colon segment, without considering disease extent. We present the Ulcerative Colitis Severity Classification and Localised Extent (UC-SCALE) algorithm, which provides a comprehensive and automated evaluation of endoscopic severity and disease extent in UC.
Methods: UC-SCALE consists of 3 main elements: 1) a quality filter selecting readable images (frames) from colonoscopy videos, 2) a scoring system assigning a MCES to each readable frame, and 3) a camera localisation algorithm assigning each frame to a location within the colon.
Article Synopsis
- Optical pooled screening (OPS) is a method that connects images of cells with genetic changes, but it previously had limitations in its ability to analyze complex data in cancer cell lines.
- The new technology, PerturbView, improves OPS by amplifying genetic barcodes for more detailed and varied phenotype analysis across different biological systems, including stem cells and immune cells.
- PerturbView has unveiled both known and new regulatory mechanisms in immune pathways, and it can be integrated with spatial transcriptomics, enhancing the potential for comprehensive studies of cellular behaviors in complex tissue environments.
The Asiatic garden beetle, Maladera formosae Brenske (AGB), has become a significant pest of commercial mint fields in northern Indiana. Larval feeding on mint roots can cause stunted growth and plant death when densities are high. Sampling approaches that provide reliable estimates of larval densities in mint have not been established, leaving farmers without the knowledge necessary to implement integrated pest management (IPM) strategies.
View Article and Find Full Text PDFSterile alpha and TIR motif containing 1 (SARM1) is an inducible NADase that localizes to mitochondria throughout neurons and senses metabolic changes that occur after injury. Minimal proteomic changes are observed upon either SARM1 depletion or activation, suggesting that SARM1 does not exert broad effects on neuronal protein homeostasis. However, whether SARM1 activation occurs throughout the neuron in response to injury and cell stress remains largely unknown.
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