Kinetics of MHC class II and transferrin receptor expression by colostral cells stimulated in vitro with concanavalin A and myelin basic protein.

The colostral cells, regarded generally as being protective, have been shown to differ in a number of membrane properties (rosetting, adherence, mobility) from the corresponding peripheral blood mononuclear cells. After in vitro stimulation with Con A or MBP 50% to 70% of the human colostral cells appeared HLA-DR positive at the first 24 h of culturing. The CD71 expression reached a maximum on culture days 2-3 coinciding with the maximal proliferative response.

Interleukin production by human colostral cells after in vitro mitogen stimulation.

Human colostral cells were pulsed with PHA, Con A, or LPS and cultivated in serum-free medium. The culture supernatants were tested for IL-1 activity in C3H/HeJ thymocyte assay and for IL-2 activity on human lymphoblasts. The IL-1 activity was the highest at the 24th h of cultivation and IL-2 activity at the 48th h of cultivation.

[Clinical and laboratory aspects of multiple plasmacytoma].

Clinical-laboratory observations were carried out on 40 patients with multiple plasmocytoma. Morbidity maximum was established to be during the fifth and sixth decade of life and that males are more frequently affected and bone manifestations are dominating initial symptoms. The anemic syndrome, the renal syndrome, erythrocyte sedimentation reaction and the data of the differential leukocyte count are discussed in details.

[Ultrastructural studies of the plasma cells in multiple myeloma].

Electron microscopic investigations of 38 patients with multiple plasmocytoma were carried out, the patients being divided into three groups according to the type of monoclonic immunoglobin in serum or urine. Light to moderate cellular-cytoplasmatic asynchronism was found as well as hypertrophic nucleoli and versatile changes in cytoplasmatic organelles of the poliferous plasmocytes. Cellular-cytoplasmatic asynchronism is discussed as a characteristic sign of neoplastic plasmatic cells.