[Inhibitors of exonuclease A5].

Over 30 compounds resembling to or being structural elements of the minimal substrate of exonuclease A5 were tested for their ability to inhibit the reaction catalyzed by this nuclease. The compounds containing less than two phosphate groups were shown to possess a low inhibitory activity, if any. CDP, double-stranded DNA and nucleoside-3',5'-diphosphates (pNp) proved to be effective exonuclease A5 inhibitors.

[Effect of yeast disintegration conditions on protein properties].

The influence of the cellular treatment with the aid of a vibrocolloidal mill on the model proteins--albumin and yeast glutelin was studied. The treatment of the model proteins in methylene chloride using the vibrocolloidal mill did not change the protein solubility. By analytical centrifugation, electrophoresis in polyacrylamide gel and gel filtration it was shown that these proteins underwent no destruction.