Structural and conformational changes in myocardial and erythrocyte actin during cardiac ischemia.

Structural and conformational changes in myocardial and erythrocyte actin during cardiac ischemia were studied by the method of fluorescence resonance energy transfer with highly selective fluorescent probes. In contrast to 15-min coronary artery occlusion, 120-min ischemia was accompanied by irreversible structural and conformational changes in the small domain of erythrocyte actin. Posttranslational changes during myocardial ischemia concerned the N- and C-terminal regions of actin and went beyond the allowed conformational fluctuations in the actin molecule without breaking the energy barrier.

Effects of verapamil and amiodarone on sympathoadrenal system and balance of excitatory and inhibitory amino acids in rat medulla oblongata.

Local injection of verapamil into ventrolateral region of the medulla oblongata triggered the release of epinephrine. Verapamil increased the total content of norepinephrine and epinephrine by 560% and decreased the content of serotonin by 46%. Verapamil had no effect on norepinephrine/epinephrine and norepinephrine/(norepinephrine+epinephrine) ratios in normal rats.

Effect of nonselective beta-adrenoblockers on the state of sympathoadrenal system and ratio of neuroactive amino acids in rat medulla oblongata.

Experiments on rats showed that injection of propranolol into the medulla oblongata increased the contents of epinephrine, norepinephrine, dopamine, and L-DOPA by 3.76, 1.4, 2.

[State of the endothelial system and expression of tissue hypoxia in children with bronchial asthma of atopic geneses].

In investigation were included 33 children with mild and moderate bronchial asthma of atopic geneses, average duration of the disease 3,5+/-1,9 years, average frequency of asthma attacks was less then once per month (night attacks 2-3 times per week), factors of initiation were dust and sharp smells. It was shown, that in atopic bronchial asthma in children, disbalance in endothelial system is developed, the content of endothelin-1, vasoconstrictor peptide of endotheliocytes, is increased by 64%. At the same time, coefficient of correlation between redox-potential NADP/NADPH and content of IgE decreases from 0,79 (p<0,01) in norm to 0,57 (p<0,05).

Cardioprotective effect of energostim during occlusion of coronary artery.

Experiments on dogs showed that energostim, a directly acting antihypoxant, injected 15 min after occlusion of the upper one-third of the left descending branch of the interventricular coronary artery produced a pronounced cardioprotective effect. The effect was confirmed by electron microscopy (evaluation the necrotic focus), biochemical tests of the heart and blood, and changes in intracardiac hemodynamics (recovery of systolic and diastolic functions). The cardioprotective effect of energostim greatly surpasses that of routine therapy applied during acute myocardium infarction.