Small RNA sequencing analysis provides novel insights into microRNA-mediated regulation of defense responses in chickpea against Fusarium wilt infection.

Small RNA sequencing analysis in two chickpea genotypes, JG 62 (Fusarium wilt-susceptible) and WR 315 (Fusarium wilt-resistant), under Fusarium wilt stress led to identification of 544 miRNAs which included 406 known and 138 novel miRNAs. A total of 115 miRNAs showed differential expression in both the genotypes across different combinations. A miRNA, Car-miR398 targeted copper chaperone for superoxide dismutase (CCS) that, in turn, regulated superoxide dismutase (SOD) activity during chickpea-Foc interaction.

American Society for Gastrointestinal Endoscopy guideline on the diagnosis and management of GERD: summary and recommendations.

This clinical practice guideline from the American Society for Gastrointestinal Endoscopy (ASGE) provides an evidence-based approach for strategies to diagnose and manage GERD. This document was developed using the Grading of Recommendations Assessment, Development, and Evaluation framework and serves as an update to the 2014 ASGE guideline on the role of endoscopy in the management of GERD. This updated guideline addresses the indications for endoscopy in patients with GERD as well as in the emerging population of patients who develop GERD after sleeve gastrectomy or peroral endoscopic myotomy.

RATS: Unsupervised manifold learning using low-distortion alignment of tangent spaces.

With the ubiquity of high-dimensional datasets in various biological fields, identifying low-dimensional topological manifolds within such datasets may reveal principles connecting latent variables to measurable instances in the world. The reliable discovery of such manifold structure in high-dimensional datasets can prove challenging, however, largely due to the introduction of distortion by leading manifold learning methods. The problem is further exacerbated by the lack of consensus on how to evaluate the quality of the recovered manifolds.