METTL3-Mediated m6A Modification of ISG15 mRNA Regulates Doxorubicin-Induced Endothelial Cell Apoptosis.

N6-adenosine methylation (m6A) of RNA is involved in the regulation of various diseases. However, its role in chemotherapy-related vascular endothelial injury has not yet been elucidated. We found that methyltransferase-like 3 (METTL3) expression was significantly reduced during doxorubicin (DOX)-induced apoptosis of vascular endothelial cells both in vivo and in vitro, and that silencing of METTL3 further intensified this process.

Leaf senescence characteristics and economic benefits of rice under alternate wetting and drying irrigation and blended use of polymer-coated and common urea.

Water-saving irrigation and the mixed application of controlled-release nitrogen fertilizer (CRNF) and common urea (CU; with a higher nitrogen release rate) have shown promise in improving rice yield with high resource use efficiency. However, the physiological mechanism underlying this effect remains largely unknown. This study involved a field experiment on rice in Jingzhou City, Central China, in 2020 and 2021.

Chronic Jet Lag Disrupts Circadian Rhythms and Induces Hyperproliferation in Murine Lacrimal Glands via ROS Accumulation.

Purpose: Chronic jet lag (CJL) is known to disrupt circadian rhythms, which regulate various physiological processes, including ocular surface homeostasis. However, the specific effects of CJL on lacrimal gland function and the underlying cellular mechanisms remain poorly understood.

Methods: A CJL model was established using C57BL/6J mice.

Leptin Receptor Deficiency-Associated Diabetes Disrupts Lacrimal Gland Circadian Rhythms and Contributes to Dry Eye Syndrome.

Purpose: This study investigated the impact of hyperglycemia in type 2 diabetes mellitus (T2DM) on the circadian rhythms and function of lacrimal glands (LGs) in contributing to dry eye syndrome. We assessed the effects of hyperglycemia on circadian gene expression, immune cell recruitment, neural activity, and metabolic pathways, and evaluated the effectiveness of insulin in restoring normal LG function.

Methods: Using a T2DM mouse model (db/db mice), circadian transcriptomic changes in LGs were analyzed through RNA sequencing over a 24-hour period.

Boosting Electrocatalytic Hydrogenation of Phenylacetylene via Accelerating Water Electrolysis on a Cr-CuO Surface.

Electrochemical alkyne reduction with HO as a hydrogen source represents a sustainable route for value-added olefin production. However, the reaction efficiency is hampered by the high voltage and low activity of Cu electrodes due to their weak adsorbed hydrogen (*H) generation property. In this article, we present the enhanced electrocatalysis of phenylacetylene to styrene over a highly dispersive Cr-doped CuO nanowire (Cr-CuO) cathode.