Blood flow distribution in necrotic versus nonnecrotic rabbit hearts.

The spatial myocardial blood flow heterogeneity of the normal heart was previously investigated by means of the standard microsphere-defined regional myocardial blood flow in nonischemic hearts. We determined the probability density functions of coronary blood flows in the rabbit heart at selected macroautoradiographic 20-microns cross-sections of the left ventricle in nonischemic as well as infarcted hearts. Macroautoradiography gave us spatial resolutions of 0.

Structure of chicken skeletal muscle troponin C at 1.78 A resolution.

The structure of chicken skeletal muscle troponin C (TnC) has been refined to an R value of 0.168, using 14 788 reflections, in the resolution range 8.0-1.

Assessment of function, perfusion, metabolism, and histology in hearts preserved with University of Wisconsin solution.

Background: University of Wisconsin solution has shown promise for prolonged cardiac preservation. This study compared the effects of 24-hour cold storage and perfusion preservation techniques by assessment of function, perfusion, metabolism, and histological changes.

Methods And Results: Three groups of rabbit hearts (n = 6 each) were evaluated: 1) control with immediate reperfusion, 2) continuous perfusion preservation, and 3) cold storage.

Refined structure of chicken skeletal muscle troponin C in the two-calcium state at 2-A resolution.

The structure of troponin C has been refined at 2A resolution to an R value of 0.172 using a total of 8,100 reflections. Troponin C has an unusual dumbbell shape with only the two C-domain high affinity sites III and IV occupied with metals, while the pair of N-domain low affinity sites I and II are devoid of metals.

Crystal structure of the promutagen O4-methylthymidine: importance of the anti conformation of the O(4) methoxy group and possible mispairing of O4-methylthymidine with guanine.

O4-Methylthymidine (O4medT) is a promutagen. To correlate its biological properties to changes in the electronic, geometric, and conformational properties of the pyrimidine base resulting from the keto to enol shift arising from methylation, an X-ray study of O4medT was undertaken. The crystal data are a = 4.