Publications by authors named "D Przygodzka"
Selection of livestock has not only led to changes in the level of their performance but also modified their behavior. As a result, within a single species, we have to deal with different behaviors of different breeds. In our study, we assumed that the different behaviors within a species are due to differences in the morphology and physiology of behavior-related systems.
View Article and Find Full Text PDFInfluence of Selective Extraction/Isolation of Heme/Hemoglobin with Hydrophobic Imidazolium Ionic Liquids on the Precision and Accuracy of Cotinine ELISA Test.
Int J Mol Sci
November 2022
Article Synopsis
- The study examined the use of ionic liquids for extracting hemoglobin from human serum to determine cotinine levels using an ELISA Kit.
- Among the tested ionic liquids, OMIM BF was identified as the most effective for extracting hemoglobin without additional reagents, achieving a 95% recovery rate.
- Quantum mechanical and molecular dynamics simulations revealed favorable interactions between the ionic liquid and hemoglobin, leading to improved accuracy of the ELISA test results by 15% to 30% based on cotinine concentration.
Postmortem carbon monoxide (CO) and hydrogen cyanide (HCN) diffusion under ambient conditions was assessed in a human cadaver model. The main objective of this study was to determine whether the postmortem diffusion of HCN and CO greatly affected the determination of HCN, carboxyhemoglobin (COHb), and carboxymyoglobin (COMb). Layered samples of blood, musculocutaneous, and muscular specimens were collected from the adult cadavers and placed in the tight chambers designed for the purpose of this experiment.
View Article and Find Full Text PDFThe routine techniques currently applied for the determination of nicotine and its major metabolites, cotinine, and -3'-hydroxycotinine, in biological fluids, include spectrophotometric, immunoassays, and chromatographic techniques. The aim of this study was to develop, and compare two new chromatographic methods high-performance liquid chromatography coupled to triple quadrupole mass spectrometry (HPLC-QQQ-MS/MS), and RP-HPLC enriched with chaotropic additives, which would allow reliable confirmation of tobacco smoke exposure in toxicological and epidemiological studies. The concentrations of analytes were determined in human plasma as the sample matrix.
View Article and Find Full Text PDFHemp seed (Cannabis sativa L.) contain large amounts of nutrients, e.g.
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