Follicular fluid nitric oxide (NO) concentrations in stimulated cycles: the relationship to embryo grading.

Objective: The microenvironment of the ovarian follicle is vital for normal oocyte development, folliculogenesis, and timely ovulation. We investigated the concentration of nitric oxide (NO) in follicular fluid, collected during oocyte retrieval after in-vitro fertilization and embryo transfer (IVF-ET) and its relationship to oocyte and embryo grading.

Methods: A total of 53 follicular fluid samples were obtained from 15 patients undergoing IVF-ET program, oocyte retrieval by transvaginal guidance was per formed approximately 34-35 h after the hCG administration in stimulated cycles.

A new gonadotropin treatment for idiopathic moderate oligoasthenospermia.

Twenty-three idiopathic oligoasthenospermic patients were subdivided into three classes according to degree of sperm concentration and typical sperm motility percentage. These patients were treated with 75 I.U.