Human breast cancer induces osteoclast activation and increases the number of osteoclasts at sites of tumor osteolysis.

The cellular mechanism through which osseous breast cancer metastases induce the focal destruction of bone (tumor osteolysis) is unknown. An athymic mouse model designed for the study of tumor osteolysis was developed and the influence of two human breast cancer tumors on bone was studied. Tumor-induced osteolysis occurred between 7 and 10 weeks after inoculation of mouse femora with MDA-MB-231 or MDA-MB-435s breast cancer cells.

The effect of high lipid diet on in vitro prostaglandin E2 and thromboxane B2 production by splenic macrophages.

Feeding animals a diet high in linoleic acid for 7 days had no effect on the in vitro production of PGE2 by unstimulated macrophages. Feeding animals the high linoleic acid diet for 30 days greatly increased the production of PGE2 by macrophages when they were unstimulated, but decreased the production of PGE2 when they were stimulated with LPS. Feeding animals a diet high in linoleic acid for 30 days increased the production of TxB2 by macrophages when they were unstimulated, but decreased the production of TxB2 when they were stimulated.

A long-term study and correlation of lymphocyte and neutrophil function in the patient with burns.

Twenty-five patients were studied to determine the effects of thermal injury on neutrophil bactericidal function and superoxide release and on lymphocyte proliferation. Neutrophils in patients with burns had depressed killing of Staphylococcus aureus for more than 150 days after burn injury, but killing of Escherichia coli returned to normal. FMLP-stimulated superoxide release by neutrophils in patients with burns was depressed for over 100 days after burn injury, whereas superoxide release by neutrophils in patients with burns stimulated with serum-opsonized zymosan was depressed for 42 days after burn injury.

The immunosuppressive effects of the in vivo administration of endotoxin as influenced by macrophages.

It is well documented that endotoxin can have immunosuppressive effects on lymphocytes and induce the production and secretion of monokines which act on the lymphocytes. To delineate the interaction between macrophages and lymphocytes more clearly, 0.15 mg of lipopolysaccharide (LPS) (E.