The fungus Aphanoascella galapagosensis affects bacterial diversity of Galapagos giant tortoise carapaces.

Aims: This study aimed to describe the bacterial microbiome associated with the carapace of three species of Galapagos giant tortoises (Chelonoidis porteri, Chelonoidis donfaustoi, and Chelonoidis vandenburghi) and determine the potential effect of the whitish lesions caused by the fungus Aphanoascella galapagosensis.

Methods And Results: We used Oxford Nanopore's MinION to evaluate the external bacterial microbiome associated with the carapaces from the aforementioned species. Taxonomic assignment was carried out by Bugseq and the bacterial communities were compared between carapaces with and without lesions using a NMDS with Bray-Curtis as the dissimilarity index.

Three Pathogens Impact Terrestrial Frogs from a High-Elevation Tropical Hotspot.

Three infectious pathogens Batrachochytrium dendrobatidis (Bd), Ranavirus (Rv) and Perkinsea (Pr) are associated with widespread and ongoing amphibian population declines. Although their geographic and host ranges vary widely, recent studies have suggested that the occurrence of these pathogens could be more common than previously thought, even in direct-developing terrestrial species traditionally considered less likely to harbor these largely aquatic pathogens. Here, we characterize Bd, Rv, and Pr infections in direct-developing terrestrial amphibians of the Pristimantis genus from the highland Ecuadorean Andes.

A coded aperture microscope for X-ray fluorescence full-field imaging.

The design and construction of an instrument for full-field imaging of the X-ray fluorescence emitted by a fully illuminated sample are presented. The aim is to produce an X-ray microscope with a few micrometers spatial resolution, which does not need to scan the sample. Since the fluorescence from a spatially inhomogeneous sample may contain many fluorescence lines, the optic which will provide the magnification of the emissions must be achromatic, i.

Fast X-ray microfluorescence imaging with submicrometer-resolution integrating a Maia detector at beamline P06 at PETRA III.

The high brilliance of third-generation synchrotron sources increases the demand for faster detectors to utilize the available flux. The Maia detector is an advanced imaging scheme for energy-dispersive detection realising dwell times per image-pixel as low as 50 µs and count rates higher than 10 × 10 s. In this article the integration of such a Maia detector in the Microprobe setup of beamline P06 at the storage ring PETRA III at the Deutsches Elektronen-Synchrotron (DESY) in Hamburg, Germany, is described.

First experimental feasibility study of VIPIC: a custom-made detector for X-ray speckle measurements.

The Vertically Integrated Photon Imaging Chip (VIPIC) was custom-designed for X-ray photon correlation spectroscopy, an application in which occupancy per pixel is low but high time resolution is needed. VIPIC operates in a sparsified streaming mode in which each detected photon is immediately read out as a time- and position-stamped event. This event stream can be fed directly to an autocorrelation engine or accumulated to form a conventional image.