Expression and characterization of scFv-6009FV in Pichia pastoris with improved ability to neutralize the neurotoxin Cn2 from Centruroides noxius.

Small single-chain variable fragments (scFv) are promising biomolecules to inhibit and neutralize toxins and to act as antivenoms. In this work, we aimed to produce a functional scFv-6009FV in the yeast Pichia pastoris, which inhibits the pure Cn2 neurotoxin and the whole venom of Centruroides noxius. We were able to achieve yields of up to 31.

mitoBK is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming.

Alterations in the function of K channels such as the voltage- and Ca-activated K channel of large conductance (BK) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, elusive. Here, we provide electrophysiological evidence for a BK splice variant localized to the inner mitochondrial membrane of murine and human BC cells (mitoBK).

Role of human Kallistatin in glucose and energy homeostasis in mice.

Objective: Kallistatin (KST), also known as SERPIN A4, is a circulating, broadly acting human plasma protein with pleiotropic properties. Clinical studies in humans revealed reduced KST levels in obesity. The exact role of KST in glucose and energy homeostasis in the setting of insulin resistance and type 2 diabetes is currently unknown.

Identification of peptides presented through the MHC-II of dendritic cells stimulated with Mycobacterium avium.

Mycobacterium avium (M. avium) represents a species of concern, because of its ability to modulate the host's innate immune response, and therefore influence trajectory of adaptative immunity. Since eradicative response against mycobacteria, and M.

The measurement of photocathode transverse energy distribution curves (TEDCs) using the transverse energy spread spectrometer (TESS) experimental system.

The minimum achievable particle beam emittance in an electron accelerator depends strongly on the intrinsic emittance of the photocathode electron source. This is measurable as the mean longitudinal and transverse energy spreads in the photoemitted electron beam (MLE and MTE respectively); consequently, MLE and MTE are notable figures of merit for photocathodes used as electron sources in particle accelerators. The overall energy spread is defined by the sum of the MTE and the MLE, and the minimization of MTE is crucial to reduce emittance and thus generate a high-brightness electron beam.