A circular, viroid-like satellite RNA (sat RNA) was detected in lucerne transient streak virus (LTSV), from lucerne in South Australia. It was larger than the previously reported sat RNA of LTSV, being similar in size and sequence homology to the 388 nucleotide sat RNA previously shown to be encapsidated by subterranean clover mottle virus (SCMoV) isolated from subterranean clover in Western Australia. This indicates that under field conditions, very similar sat RNAs can be associated with two distantly related sobemoviruses, LTSV and SCMoV.
View Article and Find Full Text PDFJ Virol Methods
April 1986
A single antibody dot immunoassay (SADI) was developed for use as a rapid, simple and sensitive technique for the direct assay of virus bound to nitrocellulose membranes. SADI has been tested for the detection of subterranean clover mottle virus (SCMoV) in small amounts of infected tissue and purified virus preparations, and the technique was found to be twelve times more sensitive than ELISA in terms of total antigen detected. DIBA, an indirect dot immunoassay, was about twice as sensitive as SADI, but the latter was more specific for the detection of SCMoV and is a simpler, more rapid assay, requiring less than three h to complete.
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